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PSME4 Antibody (PA5-89458) in IHC (P)
Immunohistochemistry analysis of PSME4 in paraffin-embedded human esophageal. Samples were incubated with PSME4 Polyclonal antibody (Product # PA5-89458) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-89458
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1634-1843 of human PSME4 (NP_0554292)
Conjugate
Form
Liquid
Concentration
1.38 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805581
Product Specific Information
Immunogen sequence: LYPHQVPLVL QVLKQTARSS SWHARYTVLT YLQTMVFYNL FIFLNNEDAV KDIRWLVISL LEDEQLEVRE MAATTLSGLL QCNFLTMDSP MQIHFEQLCK TKLPKKRKRD PGSVGDTIPS AELVKRHAGV LGLGACVLSS PYDVPTWMPQ LLMNLSAHLN DPQPIEMTVK KTLSNFRRTH HDNWQEHKQQ FTDDQLLVLT DLLVSPCYYA; Positive Samples: MCF-7, U-87MG, 293T, HeLa, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Rat liver; Cellular Location: Cytoplasm, Nucleus, Nucleus speckle, cytosol
Target Information
Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: proteasome (prosome, macropain) activator subunit 4; proteasome activator 200 kDa; Proteasome activator complex subunit 4; Proteasome activator PA200; Protein TEMO
Gene Aliases: AA409398; AU041366; KIAA0077; mKIAA0077; PA200; PSME4; TEMO
UniProt ID: (Human) Q14997, (Mouse) Q5SSW2
Entrez Gene ID: (Human) 23198, (Mouse) 103554, (Rat) 498433
DNA repair
cellular response to DNA damage stimulus
multicellular organism development
spermatogenesis
proteasomal ubiquitin-independent protein catabolic process
positive regulation of peptidase activity
cell differentiation
spermatogenesis, exchange of chromosomal proteins
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
T cell receptor signaling pathway
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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