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PSPN Antibody (500-P138-100UG) in WB
Western Blot: To detect Human Persephin by Western Blot analysis PSPN Polyclonal Antibody (Product # 500-P138-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Persephin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P138-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Persephin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929432
Product Specific Information
AA Sequence of recombinant protein: RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG.^M
^M
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Persephin. Anti-Human Persephin-specific antibody was purified by affinity chromatography employing an immobilized Human Persephin matrix.^M
^M
Sandwich ELISA: To detect Human Persephin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Persephin (500-P138Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Persephin.^M
^M
Western Blot: To detect Human Persephin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Persephin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Persephin is a disulfide-linked, homodimeric, neurotrophic factor structurally related to GDNF, artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken, and was later shown to be the preferential binding subunit for persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve oxotomy, and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and neurturin, persephin does not support the survival of peripheral neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Persephin; PSP
Gene Aliases: PSP; PSPN
UniProt ID: (Human) O60542
Entrez Gene ID: (Human) 5623
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