Invitrogen

PTCH2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

View all (10) PTCH2 antibodies

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PTCH2 Antibody in Western Blot (WB) — Western blot analysis of PTCH2 in fetal brain lysate. Samples were incubated in PTCH2 polyclonal antibody (Product # PA1-46223) using a dilution of 1:500.

PTCH2 Antibody in Immunocytochemistry (ICC/IF) — Fig 5 Ptch1/2 and co-receptors are differentially expressed in PGCs and somatic cells. a Expression of Ptch1, Ptch2, Gas1, Boc and Cdon demonstrated by immunofluorescence analyses of primary cultures of dissected E10.5 mouse GR, with co-staining of SSEA1. Representative images from three independent experiments are shown. Scale bar, 50 mum. b Single-cell suspensions from freshly-dissected GR of E10.5 GFP Stella embryos were sorted by FACS (Supplementary Fig. 4 ) and the expression of Ptch1, Ptch2, GFP and Stella in GFP-positive (PGCs) and GFP-negative (somatic cells) populations analysed by quantitative RT-PCR. Each data point represents one experiment. Values normalised to 18s rRNA were plotted with error bars representing SD ( n = 3) after unpaired t -test (* P < 0.05, ** P < 0.005, *** P < 0.001). c Immunofluorescence analyses of Gas1, Ptch2, p-Src, Boc and SSEA1 on the GR tissue sections of WT and Gas1 KO embryos at E10.5. The merged images are shown without DAPI signal for improved clarity. Representative images from three independent experiments are shown. Scale bar, 10 um. d Dot plots showing the relative fluorescence intensity values of Gas1, Ptch2, p-Src, Boc signal observed in PGCs which were normalised with the fluorescence intensity values of SSEA in each cell. Gas1 KO embryos showed a significant decrease in signal intensity in Gas1 (WT ( n = 12), KO ( n = 5), **** P < 0.0001) and p-Src (WT ( n = 7), KO ( n = 12), ** P = 0.0054) but no significant changes in Ptch

PTCH2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 6 Gas1 mediates Hh ligand reception by Ptch2. a Representative images of at least two independent immunoblotting experiments in which NIH3T3/Cas9 cells with Ptch1 KO were transfected with Myc-tagged Ptch2 expression construct and stimulated with Shh protein in serum-free medium for 40 min. Interactions of Ptch2 with endogenous Smo, Gas1 and Boc were analysed by immunoprecipitation (IP) and Western blotting (WB). IP was performed using specific antibodies as indicated and analysed by WB with respective antibodies as shown. The images in the bottom panel are from Ptch1 KO cells transfected with empty vector, which did not show any co-precipitation of endogenous Gas1 and Ptch2 regardless of Shh treatment, confirming the specificity of these antibodies (bottom panel). b A schematic diagram illustrating the protein interactions before and after Shh treatment. c NIH3T3/Cas9 cells with Gas1 KO were transfected with Myc-tagged Ptch2 and stimulated with Shh protein in serum-free medium for 40 min. IP was performed with anti-Myc antibody. Representative blots from two independent experiments are shown. d A schematic diagram illustrating the protein interactions in Gas1 KO cells. Source data are provided as a Source Data file.

PTCH2 Antibody in Western Blot (WB) — Published figure using PTCH2 polyclonal antibody (Product # PA1-46223) in Western Blot

PTCH2 Antibody — Figure 3. Abeta 1-42 not Abeta 1-40 increases the expressions of Shh signaling in hippocampal GPCs derived from WT mice. The suspended cells were incubated with the presence of 0, 0.1, 1, 10 or 100 u m of Abeta 1-42 or Abeta 1-40 for 3 days. ( A ) Shh expression with different molecular weights was shown by western blot from the cultured medium and the cells treated with Abeta 1-42 . ( B ) Shh levels were significantly increased in the conditioned medium (normalized to corresponding cell lysate) and cultured cells in a dose-dependent manner with Abeta 1-42 treatment (ANOVA test, * P < 0.05, **P < 0.01). ( C ) The expression of Shh signaling components was shown in the cells with Abeta 1-42 treatment. ( D ) The levels of Ptc1, Ptc2, Smo, Gli1 and Gli2 were significant elevated (ANOVA test, * P < 0.05, * * P < 0.01) but not significant changes of Gli3 levels with the exposure of Abeta 1-42 (ANOVA test, P > 0.05). ( E ) Shh expression was shown from the conditioned medium and GPCs treated with Abeta 1-40 . ( F ) Shh levels were not significantly increased in the conditioned medium (normalized to corresponding cell lysate) and the cultured GPCs with the Abeta 1-40 treatment (ANOVA test, P > 0.05). ( G ) The expression of Shh signaling component molecules was shown in the GPC lysate with the Abeta 1-40 treatment. ( H ) The levels of the components were not significantly changed with the Abeta 1-40 treatment. ( I ) Western blot showed Ptc1 expression with the exogenous Shh applicat {TM}

Product Details

PA1-46223

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:500	-
Immunohistochemistry (Frozen) (IHC (F))	1:10-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:500	View 1 publication 1 publication
ELISA (ELISA)	1:100-1:2,000	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Residues 226-235 of the human PTCH2 protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2174052

Target Information

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas. PTCH2 has a frameshift mutation resulting in a truncated PTCH2 protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Nature communications

Kim Y,Lee J,Seppala M,Cobourne MT,Kim SH

PA1-46223 was used in Immunohistochemistry-immunofluorescence to identify a divergent Hedgehog signal pathway mediated by Ptch2 and provide a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche.

Fri Apr 24 00:00:00 EDT 2020

Deficiency of patched 1-induced Gli1 signal transduction results in astrogenesis in Swedish mutated APP transgenic mice.

Human molecular genetics

He P,Staufenbiel M,Li R,Shen Y

PA1-46223 was used in western blot to report a regulatory role for the Ptc1-Gli1 complex in adult neural circuit integrity of the brain

Mon Dec 15 00:00:00 EST 2014

Bioinformatics

Protein Aliases: patched homolog 2; Protein patched homolog 2; PTC2; RP11-269F19.8

Gene Aliases: PTC2; PTCH2; UNQ560/PRO1121/PRO57079

UniProt ID: (Human) Q9Y6C5, (Mouse) O35595

Entrez Gene ID: (Human) 8643, (Mouse) 19207

cell fate determination epidermis development epidermal cell fate specification hair cycle skin development positive regulation of epidermal cell differentiation signal transduction negative regulation of smoothened signaling pathway

Process(es)

smoothened binding hedgehog receptor activity hedgehog family protein binding