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          • Primary Antibodies ›
          • PTCRA Antibodies

          Invitrogen

          PTCRA Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (3) PTCRA antibodies

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          Cite PTCRA Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          PTCRA Antibody in Western Blot (WB)
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          PTCRA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          PTCRA Antibody (PA5-95578) in WB

          Western blot analysis of PTCRA in, Lane 1: human A375 whole cell lysate, Lane 2: human HL-60 whole cell lysate, Lane 3: human CCRM-CEM whole cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PTCRA Antibody in Western Blot (WB)
          PTCRA Antibody in Western Blot (WB)
          PTCRA Polyclonal Antibody

          Product Details

          PA5-95578

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human PTCRA recombinant protein (Position: T24-T143).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807380

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human A375 whole cell, human HL-60 whole cell, human CCRM-CEM whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Pre T-cell antigen receptor alpha; pre-T-cell receptor alpha chain; pT-alpha; pT-alpha-TCR; unnamed protein product

          View more View less

          Gene Aliases: IMD126; PT-ALPHA; PTA; PTCRA

          View more View less

          UniProt ID: (Human) Q6ISU1

          View more View less

          Entrez Gene ID: (Human) 171558

          View more View less

          Function(s)
          protein binding
          Process(es)
          alpha-beta T cell differentiation negative regulation of thymocyte apoptotic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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