Invitrogen

PTPN22 Polyclonal Antibody

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PTPN22 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PTPN22 in human lymph node using a PTPN22 Polyclonal Antibody (Product # PA5-52132) shows stark ctoplasmic positivity in a subset of germinal center cells and non-germinal center cells.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:20-1:50

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PTPN22

Conjugate

Unconjugated

Form

Liquid

Concentration

Lot-Specific

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2646124

Product Specific Information

Immunogen sequence: SEISAKEELV LHPAKSSTSF DFLELNYSFD KNADTTMKWQ TKAFPIVGEP LQKHQSLDLG SLLFEGCSNS KPVNAAGRYF NSKVPITRTK S

Highest antigen sequence identity to the following orthologs: Mouse - 57%, Rat - 59%.

Target Information

The protein tyrosine phosphatase PTPN22 (PTP22, LYP, PEP, formerly PTPN8) is a genetic variant that confers risk of developing diverse human autoimmune diseases such as type 1 diabetes and rheumatoid arthritis. The minor allele of a missense SNP in PTPN22 encodes a hematopoietic-specific protein tyrosine phosphatase also known as ""Lyp."" The risk allele is present in about 17% of Caucasian individuals from the general population and in approximately 28% of Caucasian individuals with rheumatoid arthritis; it is thought to disrupt the P1 proline-rich motif that is important for interaction with the Src homology-3 (SH3) domain of CSK (cytoplasmic tyrosine kinase), potentially altering the normal functions of these proteins as negative regulators of T cell activation. The interaction between CSK and PTPN22 is highly specific and it is speculated that PTPN22 may be an effector and/or regulator of CSK in T cells and other hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; LyP; PEP; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; PTPN22; PTPN8; Tyrosine-protein phosphatase non-receptor type 22

Gene Aliases: LYP; LYP1; LYP2; PEP; PTPN22; PTPN8

UniProt ID: (Human) Q9Y2R2

Entrez Gene ID: (Human) 26191

Molecular Function: hydrolase phosphatase protein phosphatase