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Invitrogen
PTPN22 Polyclonal Antibody
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FIGURE: 1 / 4
PTPN22 Antibody (PA5-79896) in IHC (P)
Immunohistochemistry analysis of PTPN22 on paraffin-embedded rat lymphaden tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with PTPN22 polyclonal antibody (Product# PA5-79896) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Deve... View More
Product Details
PA5-79896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse PTPN22 recombinant protein (Position: M1-Q309).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat thymus tissue, human SW579 whole cell, human HepG2 whole cell, human placenta tissue, human Hela whole cell, human SW620 whole cell. IHC: mouse spleen tissue, rat lymphaden tissue.
Target Information
The protein tyrosine phosphatase PTPN22 (PTP22, LYP, PEP, formerly PTPN8) is a genetic variant that confers risk of developing diverse human autoimmune diseases such as type 1 diabetes and rheumatoid arthritis. The minor allele of a missense SNP in PTPN22 encodes a hematopoietic-specific protein tyrosine phosphatase also known as "Lyp." The risk allele is present in about 17% of Caucasian individuals from the general population and in approximately 28% of Caucasian individuals with rheumatoid arthritis; it is thought to disrupt the P1 proline-rich motif that is important for interaction with the Src homology-3 (SH3) domain of CSK (cytoplasmic tyrosine kinase), potentially altering the normal functions of these proteins as negative regulators of T cell activation. The interaction between CSK and PTPN22 is highly specific and it is speculated that PTPN22 may be an effector and/or regulator of CSK in T cells and other hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; LyP; PEP; PEST domain-enriched tyrosine phosphatase; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 22
Gene Aliases: 70zpep; LYP; LYP1; LYP2; PEP; PTPN22; PTPN8
UniProt ID: (Human) Q9Y2R2, (Mouse) P29352
Entrez Gene ID: (Human) 26191, (Rat) 295338, (Mouse) 19260
protein dephosphorylation
biological_process
T cell differentiation
regulation of natural killer cell proliferation
peptidyl-tyrosine dephosphorylation
phosphoanandamide dephosphorylation
T cell receptor signaling pathway
regulation of T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
immune system process
autophagy
dephosphorylation
negative regulation of autophagy
positive regulation of gene expression
negative regulation of gene expression
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
positive regulation of toll-like receptor 3 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
negative regulation of JUN kinase activity
regulation of innate immune response
regulation of B cell receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to muramyl dipeptide
negative regulation of interleukin-6 secretion
regulation of NIK/NF-kappaB signaling
positive regulation of protein K63-linked ubiquitination
positive regulation of interferon-gamma secretion
negative regulation of p38MAPK cascade
negative regulation of interleukin-8 secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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