Invitrogen

PTPN22 Polyclonal Antibody

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PTPN22 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PTPN22 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PTPN22 polyclonal antibody (Product # PA5-99219) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

PTPN22 Antibody in Western Blot (WB) — Western blot analysis of PTPN22 in HeLa cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with PTPN22 polyclonal antibody (Product # PA5-99219).

Product Details

PA5-99219

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PTPN22, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2818152

Target Information

The protein tyrosine phosphatase PTPN22 (PTP22, LYP, PEP, formerly PTPN8) is a genetic variant that confers risk of developing diverse human autoimmune diseases such as type 1 diabetes and rheumatoid arthritis. The minor allele of a missense SNP in PTPN22 encodes a hematopoietic-specific protein tyrosine phosphatase also known as "Lyp." The risk allele is present in about 17% of Caucasian individuals from the general population and in approximately 28% of Caucasian individuals with rheumatoid arthritis; it is thought to disrupt the P1 proline-rich motif that is important for interaction with the Src homology-3 (SH3) domain of CSK (cytoplasmic tyrosine kinase), potentially altering the normal functions of these proteins as negative regulators of T cell activation. The interaction between CSK and PTPN22 is highly specific and it is speculated that PTPN22 may be an effector and/or regulator of CSK in T cells and other hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; LyP; PEP; PEST domain-enriched tyrosine phosphatase; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 22

Gene Aliases: 70zpep; LYP; LYP1; LYP2; PEP; PTPN22; PTPN8

UniProt ID: (Human) Q9Y2R2, (Mouse) P29352

Entrez Gene ID: (Human) 26191, (Rat) 295338, (Mouse) 19260

Function(s)