Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-35158
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:10-1:50 | - |
Immunocytochemistry (ICC/IF) |
1:10-1:50 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / Ig |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A, Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.09% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2552468 |
Product Specific Information
This antibody is predicted to react with mouse, porcine and rat based on sequence homology.
Target Information
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1
Gene Aliases: Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1
UniProt ID: (Human) P17947, (Mouse) P17433
Entrez Gene ID: (Human) 6688, (Mouse) 20375
Molecular Function:
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
lymphoid progenitor cell differentiation
transcription from RNA polymerase II promoter
lymphocyte differentiation
erythrocyte differentiation
macrophage differentiation
granulocyte differentiation
somatic stem cell population maintenance
myeloid dendritic cell differentiation
histone H3 acetylation
hypermethylation of CpG island
negative regulation of MHC class II biosynthetic process
regulation of erythrocyte differentiation
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
anatomical structure regression
cellular response to ethanol
negative regulation of histone H4 acetylation
apoptotic process involved in patterning of blood vessels
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
vasculature development
myeloid leukocyte differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
RNA binding
protein binding
NFAT protein binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
sequence-specific DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support