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PUM1 Antibody (PA5-30327) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PUM1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PUM1 Polyclonal Antibody (Product # PA5-30327) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-30327
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 902 and 1186 of Human Pumilio 1
Conjugate
Form
Liquid
Concentration
1.49 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547801
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (97%), Chicken (98%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HsPUM; pumilio 1; Pumilio homolog 1; pumilio RNA-binding family member 1; pumilio-1
Gene Aliases: AA517475; HSPUM; KIAA0099; mKIAA0099; PUM1; PUMH; PUMH1; PUML1; Pumm
UniProt ID: (Human) Q14671, (Mouse) Q80U78
Entrez Gene ID: (Human) 9698, (Mouse) 80912
spermatogenesis
adult locomotory behavior
posttranscriptional regulation of gene expression
posttranscriptional gene silencing
regulation of mRNA stability
stem cell differentiation
regulation of cell cycle
regulation of chromosome segregation
regulation of gene silencing by miRNA
mRNA destabilization
positive regulation of RIG-I signaling pathway
positive regulation of gene silencing by miRNA
regulation of translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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