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Proteintech

PUMA Polyclonal Antibody

57 References
View all (20) PUMA alpha antibodies

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Product Details

55120-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 50 publications 50 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunoprecipitation (IP)

0.5-4.0 µg
-

Miscellaneous PubMed (Misc)

-
View 2 publications 2 publications

Not applicable (N/A)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Amount

45 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol, 0.1M glycine

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.

Target Information

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bcl-2 binding component 3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis; p53-induced BH3-only protein; p53-upregulated modulator of apoptosis; unnamed protein product

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Gene Aliases: BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1

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UniProt ID: (Human) Q96PG8, (Rat) Q80ZG6, (Mouse) Q99ML1

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Entrez Gene ID: (Human) 27113, (Rat) 317673, (Mouse) 170770

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Function(s)
protein binding ATPase binding
Process(es)
release of cytochrome c from mitochondria apoptotic process cellular response to DNA damage stimulus determination of adult lifespan positive regulation of protein complex assembly response to endoplasmic reticulum stress intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of apoptotic process positive regulation of neuron apoptotic process fibroblast apoptotic process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress lymphocyte apoptotic process positive regulation of lymphocyte apoptotic process T cell apoptotic process positive regulation of T cell apoptotic process positive regulation of thymocyte apoptotic process cellular response to hypoxia cellular response to ionizing radiation positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway intrinsic apoptotic signaling pathway execution phase of apoptosis positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of establishment of protein localization to mitochondrion positive regulation of IRE1-mediated unfolded protein response positive regulation of fibroblast apoptotic process positive regulation of intrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic process brain development glial cell differentiation positive regulation of protein homooligomerization negative regulation of endoplasmic reticulum calcium ion concentration negative regulation of growth release of sequestered calcium ion into cytosol positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway toxin transport positive regulation of cysteine-type endopeptidase activity negative regulation of cell growth intrinsic apoptotic signaling pathway by p53 class mediator
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