Proteintech

PUMA Polyclonal Antibody

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PUMA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 55120-1-AP ( PUMA Antibody) at dilution of 1:200 (under 40x lens).

PUMA Antibody in Western Blot (WB) — Mouse and rat heart tissue were subjected to SDS PAGE followed by western blot with 55120-1-AP (PUMA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PUMA Antibody in Immunoprecipitation (IP) — IP result of anti-PUMA (IP:55120-1-AP, 4ug; Detection:55120-1-AP 1:500) with mouse heart tissue lysate 3200ug.

Product Details

55120-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 18 publications 18 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.

Target Information

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ginseng metabolite protopanaxadiol interferes with lipid metabolism and induces endoplasmic reticulum stress and p53 activation to promote cancer cell death.

Phytotherapy research : PTR

Jin HR,Du CH,Wang CZ,Yuan CS,Du W

Fri Mar 01 00:00:00 EST 2019

Sigma-1 receptor protects against endoplasmic reticulum stress-mediated apoptosis in mice with cerebral ischemia/reperfusion injury.

Apoptosis : an international journal on programmed cell death

Zhao X,Zhu L,Liu D,Chi T,Ji X,Liu P,Yang X,Tian X,Zou L

Fri Feb 01 00:00:00 EST 2019

Involvement of NLRP3 inflammasome in methamphetamine-induced microglial activation through miR-143/PUMA axis.

Toxicology letters

Du L,Shen K,Bai Y,Chao J,Hu G,Zhang Y,Yao H

Fri Feb 01 00:00:00 EST 2019

SIRT1 downregulation mediated Manganese-induced neuronal apoptosis through activation of FOXO3a-Bim/PUMA axis.

The Science of the total environment

Zhao X,Liu Y,Zhu G,Liang Y,Liu B,Wu Y,Han M,Sun W,Han Y,Chen G,Jiang J

Tue Jan 01 00:00:00 EST 2019

Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide.

Environmental pollution (Barking, Essex : 1987)

Ma J,Li X

Thu Nov 01 00:00:00 EDT 2018

Cattle Encephalon Glycoside and Ignotin Reduce Early Brain Injury and Cognitive Dysfunction after Subarachnoid Hemorrhage in Rats.

Neuroscience

Ma K,Li R,Zhao H,Qu J,Mu N,Liu X,Wang S,Yang C,Feng H,Tan L,Li F

Sat Sep 15 00:00:00 EDT 2018

Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells.

Toxicology and applied pharmacology

Tu W,Zhang Q,Liu Y,Han L,Wang Q,Chen P,Zhang S,Wang A,Zhou X

Tue May 15 00:00:00 EDT 2018

Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells.

Oncology letters

Tu S,Zhang XL,Wan HF,Xia YQ,Liu ZQ,Yang XH,Wan FS

Sun Apr 01 00:00:00 EDT 2018

Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells.

Oncology letters

Tu S,Zhang XL,Wan HF,Xia YQ,Liu ZQ,Yang XH,Wan FS

Sun Apr 01 00:00:00 EDT 2018

SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.

eLife

Fang Z,Cao B,Liao JM,Deng J,Plummer KD,Liao P,Liu T,Zhang W,Zhang K,Li L,Margolin D,Zeng SX,Xiong J,Lu H

Fri Mar 16 00:00:00 EDT 2018

Vitamin K2 Induces Mitochondria-Related Apoptosis in Human Bladder Cancer Cells via ROS and JNK/p38 MAPK Signal Pathways.

PloS one

Duan F,Yu Y,Guan R,Xu Z,Liang H,Hong L

Fri Aug 18 00:00:00 EDT 2017

Vitamin K2 Induces Mitochondria-Related Apoptosis in Human Bladder Cancer Cells via ROS and JNK/p38 MAPK Signal Pathways.

PloS one

Duan F,Yu Y,Guan R,Xu Z,Liang H,Hong L

Fri Aug 18 00:00:00 EDT 2017

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Nature communications

Yao Y,Lu Q,Hu Z,Yu Y,Chen Q,Wang QK

Tue Jul 25 00:00:00 EDT 2017

Long intragenic non-coding RNA lincRNA-p21 suppresses development of human prostate cancer.

Cell proliferation

Wang X,Ruan Y,Wang X,Zhao W,Jiang Q,Jiang C,Zhao Y,Xu Y,Sun F,Zhu Y,Xia S,Xu D

Sat Apr 01 00:00:00 EDT 2017

Nuclear retention of the lncRNA SNHG1 by doxorubicin attenuates hnRNPC-p53 protein interactions.

EMBO reports

Shen Y,Liu S,Fan J,Jin Y,Tian B,Zheng X,Fu H

Sat Apr 01 00:00:00 EDT 2017

Inhibitory effect and mechanism of exogenous mammalian sterile 20-like kinase 1 on the growth of human colorectal cancer.

Oncology letters

Wu J,Yang X,Lu H,Liu L,Xu B,Zheng S,Yu B,Jie K,Wan F

Sat Apr 01 00:00:00 EDT 2017

The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.

PLoS biology

Liu H,Li X,Ning G,Zhu S,Ma X,Liu X,Liu C,Huang M,Schmitt I,Wüllner U,Niu Y,Guo C,Wang Q,Tang TS

Tue Nov 01 00:00:00 EDT 2016

Inhibition of Drp1 mitochondrial translocation provides neural protection in dopaminergic system in a Parkinson's disease model induced by MPTP.

Scientific reports

Filichia E,Hoffer B,Qi X,Luo Y

Tue Sep 13 00:00:00 EDT 2016

Dopaminergic neuron-specific deletion of p53 gene is neuroprotective in an experimental Parkinson's disease model.

Journal of neurochemistry

Qi X,Davis B,Chiang YH,Filichia E,Barnett A,Greig NH,Hoffer B,Luo Y

Thu Sep 01 00:00:00 EDT 2016

PUMA and survivin are involved in the apoptosis of HepG2 cells induced by microcystin-LR via mitochondria-mediated pathway.

Chemosphere

Ma J,Feng Y,Liu Y,Li X

Mon Aug 01 00:00:00 EDT 2016

Exogenous p53 upregulated modulator of apoptosis (PUMA) decreases growth of lung cancer A549 cells.

Asian Pacific journal of cancer prevention : APJCP

Liu CJ,Zhang XL,Luo DY,Zhu WF,Wan HF,Yang JP,Yang XJ,Wan FS

Mon Dec 14 00:00:00 EST 2015

Exogenous p53 upregulated modulator of apoptosis (PUMA) decreases growth of lung cancer A549 cells.

Asian Pacific journal of cancer prevention : APJCP

Liu CJ,Zhang XL,Luo DY,Zhu WF,Wan HF,Yang JP,Yang XJ,Wan FS

Mon Dec 14 00:00:00 EST 2015

The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer.

The Journal of biological chemistry

Li H,Liu Q,Wang Z,Fang R,Shen Y,Cai X,Gao Y,Li Y,Zhang X,Ye L

Fri Sep 11 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Bcl-2 binding component 3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis

Gene Aliases: BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1

UniProt ID: (Human) Q96PG8, (Rat) Q80ZG6, (Mouse) Q99ML1

Entrez Gene ID: (Human) 27113, (Rat) 317673, (Mouse) 170770

Function(s)