Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 18 publications 18 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:500 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Miscellaneous PubMed (Misc) |
- | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Peptide |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.3 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bcl-2 binding component 3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis
Gene Aliases: BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1
UniProt ID: (Human) Q96PG8, (Rat) Q80ZG6, (Mouse) Q99ML1
Entrez Gene ID: (Human) 27113, (Rat) 317673, (Mouse) 170770
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