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Invitrogen
PUMA alpha Polyclonal Antibody
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Product Details
PA1-4277
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: E(101) Q H L E S P V P S A P G A L A G(117)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-4277 detects alpha- and beta-PUMA from human samples.
PA1-4277 has been successfully used in Western blot procedures. By Western blot this antibody recognizes both forms of the PUMA protein found at ~20 kDa and ~36 kDa in transfected U20S cells.
PA1-4277 immunizing peptide corresponds to amino acid residues E(101) Q H L E S P V P S A P G A L A G(117) of the human PUMA-a form of the PUMA protein.
Target Information
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis; p53-induced BH3-only protein; p53-upregulated modulator of apoptosis; unnamed protein product
Gene Aliases: BBC3; JFY-1; JFY1; PUMA
UniProt ID: (Human) Q96PG8
Entrez Gene ID: (Human) 27113
release of cytochrome c from mitochondria
apoptotic process
cellular response to DNA damage stimulus
determination of adult lifespan
positive regulation of protein complex assembly
response to endoplasmic reticulum stress
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
fibroblast apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
lymphocyte apoptotic process
positive regulation of lymphocyte apoptotic process
T cell apoptotic process
positive regulation of T cell apoptotic process
positive regulation of thymocyte apoptotic process
cellular response to hypoxia
cellular response to ionizing radiation
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of establishment of protein localization to mitochondrion
positive regulation of IRE1-mediated unfolded protein response
positive regulation of fibroblast apoptotic process
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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