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Invitrogen
PXR Polyclonal Antibody
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FIGURE: 1 / 2
PXR Antibody (PA5-102431) in ICC/IF
Immunocytochemistry analysis of PXR in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PXR polyclonal antibody (Product # PA5-102431) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102431
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NR1I2(Accession O75469), corresponding to amino acid residues L348-T398.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NR1I2.
Target Information
This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group 1, member 2; nuclear receptor subfamily 1, group I, member 2; ONR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR 1; PAR 2; PAR q; pregnane X nuclear receptor variant 2; Pregnane X receptor; pregnane X receptor (nuclear receptor sub family 1, group I, member 2); Steroid and xenobiotic receptor; SXR
Gene Aliases: BXR; mPXR; NR1I2; ONR1; PAR; PAR1; PAR2; PARq; PRR; PXR; PXR.1; PXR.2; PXR1; SAR; SXR
UniProt ID: (Human) Q9UJ22, (Rat) Q9R1A7, (Mouse) O54915
Entrez Gene ID: (Human) 8856, (Rat) 84385, (Mouse) 18171
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
drug binding
zinc ion binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
metal ion binding
transcription, DNA-templated
xenobiotic metabolic process
positive regulation of gene expression
intracellular receptor signaling pathway
exogenous drug catabolic process
xenobiotic transport
steroid hormone mediated signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
drug export
transcription initiation from RNA polymerase II promoter
signal transduction
steroid metabolic process
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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