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FIGURE: 1 / 6
PYGO2 Antibody (PA5-31553) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PYGO2 was performed in HCT 116 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PYGO2 Polyclonal Antibody (Product # PA5-31553) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-31553
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length mouse Pygopus 2 Recombinant protein.
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549026
Product Specific Information
Recommended positive controls: NIH-3T3.
Predicted reactivity: Human (96%), Mouse (100%), Rat (99%), Pig (97%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The PYGO2 gene encodes pygopus family PHD finger 2, a key component of the Wnt signaling pathway, which is critical for embryonic development, cell proliferation, and differentiation. PYGO2 operates as a chromatin remodeling factor, interacting with the beta-catenin-LEF/TCF transcriptional complex, facilitating the transcription of Wnt target genes by recruiting chromatin-modifying enzymes. This activity is vital for maintaining the balance between stem cell renewal and differentiation in various tissues, including the brain and the skin. Additionally, PYGO2 plays significant roles in spermatogenesis and hematopoiesis by modulating gene expression necessary for these processes. Dysregulation of PYGO2 expression can lead to developmental abnormalities and is often implicated in cancer progression, indicating its potential as a biomarker for tumorigenic stem cell-like properties and metastatic behavior. Research continues to explore PYGO2's mechanisms and interactions to understand its contributions to development and its implications in disease states, particularly in cancer biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mpygo2; pygopus 2; Pygopus homolog 2
Gene Aliases: 1190004M21Rik; PP7910; PYGO2; RGD1308535
UniProt ID: (Human) Q9BRQ0
Entrez Gene ID: (Human) 90780, (Rat) 295251, (Mouse) 68911
in utero embryonic development
kidney development
lens development in camera-type eye
spermatid nucleus differentiation
brain development
post-embryonic development
mammary gland development
regulation of mammary gland epithelial cell proliferation
regulation of histone acetylation
positive regulation of chromatin binding
developmental growth
regulation of histone H3-K4 methylation
palate development
canonical Wnt signaling pathway
beta-catenin-TCF complex assembly
spermatid development
Wnt signaling pathway
regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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