|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the mouse Pannexin 1 protein (Accession# NP_062355), which is identical to rat and 92% homologous to human sequence.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2-4 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dual Oxidase 2 (Duox2) Regulates Pannexin 1-mediated ATP Release in Primary Human Airway Epithelial Cells via Changes in Intracellular pH and Not H2O2 Production.
488100 was used in western blot to determine the role of Duox2 and how it regulates Pannexin 1-mediated ATP release in primary human airway epithelial cells via changes in intracellular pH and not H202 production
|Krick S,Wang J,St-Pierre M,Gonzalez C,Dahl G,Salathe M||The Journal of biological chemistry (291:6423)||2016|
Neuronal pannexin-1 channels are not molecular routes of water influx during spreading depolarization-induced dendritic beading.
488100 was used in immunohistochemistry to study the spreading depolarization-induced dendritic beading where neuronal pannexin-1 channels are not molecular routes of water influx
|Sword J,Croom D,Wang PL,Thompson RJ,Kirov SA||Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (null:null)||2016|
Anoxia-induced NMDA receptor activation opens pannexin channels via Src family kinases.
488100 was used in immunohistochemistry to identify mechanisms of Panx activation.
|Weilinger NL,Tang PL,Thompson RJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:12579)||2012|
A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus.
488100 was used in immunocytochemistry and western blot to study pannexin-1 and it role in the activation of innate immune cells of the choroid plexus
|Maslieieva V,Thompson RJ||Channels (Austin, Tex.) (8:131)||2014|
Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats.
488100 was used in immunohistochemistry - frozen section and western blot to discuss the role of NLRP/NALP during central nervous system injury.
|Mawhinney LJ,de Rivero Vaccari JP,Dale GA,Keane RW,Bramlett HM||BMC neuroscience (12:null)||2011|