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Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Sea urchin, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified parvalbumin from rat skeletal muscle.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Immunoprecipitation (IP)||5 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-933 detects parvalbumin protein in human and rat samples.
PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum.
Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle.
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin.
Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aging somatosensory cortex displays increased density of WFA-binding perineuronal nets associated with GAD-negative neurons.
PA1-933 was used in immunohistochemistry - paraffin section to test if perineuronal nets are altered during early aging
|Karetko-Sysa M,Skangiel-Kramska J,Nowicka D||Neuroscience (277:734)||2014|
Early life stress disrupts social behavior and prefrontal cortex parvalbumin interneurons at an earlier time-point in females than in males.
PA1-933 was used in western blot to study temporal differences in the behavioural and neurological effects of early life stress in males and females
|Holland FH,Ganguly P,Potter DN,Chartoff EH,Brenhouse HC||Neuroscience letters (566:131)||2014|
Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function.
PA1-933 was used in western blot to study the effects of ageing on muscle quality and sarcoplasmic reticulum Ca(2+) release.
|Russ DW,Grandy JS,Toma K,Ward CW||Acta physiologica (Oxford, England) (201:391)||2011|
Cholinergic neurons excite cortically projecting basal forebrain GABAergic neurons.
PA1-933 was used in immunohistochemistry to study the excitation of cortically projecting basal forebrain GABAergic neurons by adjacent cholinegic neurons
|Yang C,McKenna JT,Zant JC,Winston S,Basheer R,Brown RE||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:2832)||2014|
Ataxic Guillain-Barré syndrome and acute sensory ataxic neuropathy form a continuous spectrum.
PA1-933 was used in immunohistochemistry to study the ataxic Guillain-Barr syndrome and acute sensory ataxic neuropathy
|Ito M,Matsuno K,Sakumoto Y,Hirata K,Yuki N||Journal of neurology, neurosurgery, and psychiatry (82:294)||2011|
Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by growth factors. Laboratory investigation.
PA1-933 was used in immunohistochemistry to investigate the effect of growth factors on neuron proliferation and growth after ischemia
|Yoshikawa G,Momiyama T,Oya S,Takai K,Tanaka J,Higashiyama S,Saito N,Kirino T,Kawahara N||Journal of neurosurgery (113:835)||2010|
Parvalbumin-containing neurons, perineuronal nets and experience-dependent plasticity in murine barrel cortex.
PA1-933 was used in immunohistochemistry to study the neuronal plasticity in murine barrel cortex during development
|Nowicka D,Soulsby S,Skangiel-Kramska J,Glazewski S||The European journal of neuroscience (30:2053)||2009|
Endonuclease G expression in thalamic reticular nucleus after global cerebral ischemia.
PA1-933 was used in immunohistochemistry to investigate the changes of endonuclease G in thalamic reticular nucleus neurons after ischemia
|Nielsen M,Zimmer J,Diemer NH||Experimental brain research (190:81)||2008|
Nogo-A expression after focal ischemic stroke in the adult rat.
PA1-933 was used in immunohistochemistry to detect the expression of Nogo-A protein after ischemic stroke in rat
|Cheatwood JL,Emerick AJ,Schwab ME,Kartje GL||Stroke; a journal of cerebral circulation (39:2091)||2008|
Parvalbumin (calcium binding protein); Parvalbumin alpha; PV
D22S749; PALB1; Pva; PVALB