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Invitrogen
Parvalbumin Polyclonal Antibody
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FIGURE: 1 / 35
Parvalbumin Antibody (PA1-933) in ICC/IF
Immunofluorescent analysis of MAP2 (green) and parvalbumin (red) on rat primary Hippocampal neurons (E18) (Product # A15587) cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-... View More
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.jpg?time=20220826?time=20220826 "Parvalbumin Antibody (PA1-933) in WB")
Product Details
PA1-933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse,
Rat,
Sea urchin,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified parvalbumin from rat skeletal muscle.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M sodium phosphate, pH 7.0, with 20mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-933 detects parvalbumin protein in human and rat samples.
PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum.
Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:9704; MGC116759; Parvalbumin (calcium binding protein); Parvalbumin alpha
Gene Aliases: D22S749; PALB1; Pva; PVALB
UniProt ID: (Human) P20472, (Rat) P02625
Entrez Gene ID: (Human) 5816, (Rat) 25269
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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