Proteintech

Paxillin Polyclonal Antibody

Paxillin Antibody in Immunocytochemistry (ICC/IF)

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Paxillin Antibody (10029-1-IG) in ICC/IF

Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells using 10029-1-IG (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). The cytoskeleton was labelled in red with 66031-1-IG (alpha tubulin).

Paxillin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Paxillin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10029-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma.

Bone research

Luo M,Huang M,Yang N,Zhu Y,Huang P,Xu Z,Wang W,Cai L

Mon May 29 00:00:00 UTC 2023

In Vivo and In Vitro Fibroblasts' Behavior and Capsular Formation in Correlation with Smooth and Textured Silicone Surfaces.

Aesthetic plastic surgery

Huang SQ,Chen Y,Zhu Q,Zhang YM,Lei ZY,Zhou X,Fan DL

Wed Jun 01 00:00:00 UTC 2022

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Cell death & disease

Hou J,Xu M,Gu H,Pei D,Liu Y,Huang P,Chang H,Cui H

Thu Jan 13 00:00:00 UTC 2022

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Molecular cell

Arango D,Sturgill D,Yang R,Kanai T,Bauer P,Roy J,Wang Z,Hosogane M,Schiffers S,Oberdoerffer S

Thu Aug 04 00:00:00 UTC 2022

DDX17 promotes the growth and metastasis of lung adenocarcinoma.

Cell death discovery

Liu X,Li L,Geng C,Wen S,Zhang C,Deng C,Gao X,Zhang G,He QY,Liu L

Sat Oct 22 00:00:00 UTC 2022

DDX17 promotes the growth and metastasis of lung adenocarcinoma.

Cell death discovery

Liu X,Li L,Geng C,Wen S,Zhang C,Deng C,Gao X,Zhang G,He QY,Liu L

Sat Oct 22 00:00:00 UTC 2022

Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.

Molecular neurobiology

Yao Q,Wang H,Chen H,Li Z,Jiang Y,Li Z,Wang J,Xing Y,Liu F,Yu D,Yin S

Sat Jan 01 00:00:00 UTC 2022

Structure-based discovery of a specific SHP2 inhibitor with enhanced blood-brain barrier penetration from PubChem database.

Bioorganic chemistry

Ma Y,Li WY,Sun T,Zhang L,Lu XH,Yang B,Wang RL

Fri Apr 01 00:00:00 UTC 2022

TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis.

Nature communications

Meng Z,Li Z,Xie M,Yu H,Jiang L,Yao X

Thu Sep 29 00:00:00 UTC 2022

Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.

Acta pharmacologica Sinica

Li K,Liu YY,Lv XF,Lin ZM,Zhang TT,Zhang FR,Guo JW,Hong Y,Liu X,Lin XC,Zhou JG,Wu QQ,Liang SJ,Shang JY

Thu Apr 01 00:00:00 UTC 2021

SphK1-driven autophagy potentiates focal adhesion paxillin-mediated metastasis in colorectal cancer.

Cancer medicine

Wu JN,Lin L,Luo SB,Qiu XZ,Zhu LY,Chen D,Wei ED,Fu ZH,Qin MB,Liang ZH,Huang JA,Liu SQ

Wed Sep 01 00:00:00 UTC 2021

Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.

Science signaling

Anastasiou O,Hadjisavva R,Skourides PA

Tue Feb 25 00:00:00 UTC 2020

Surface changes of nanotopography by carbon ion implantation to enhance the biocompatibility of silicone rubber: an in vitro study of the optimum ion fluence and adsorbed protein.

Journal of materials science. Materials in medicine

Li X,Zhou X,Chen Y,Yu S,Chen X,Xia X,Shi X,Zhang Y,Fan D

Fri Sep 15 00:00:00 UTC 2017

Surface changes of nanotopography by carbon ion implantation to enhance the biocompatibility of silicone rubber: an in vitro study of the optimum ion fluence and adsorbed protein.

Journal of materials science. Materials in medicine

Li X,Zhou X,Chen Y,Yu S,Chen X,Xia X,Shi X,Zhang Y,Fan D

Fri Sep 15 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: FLJ16691; hCG_1778014; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134

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Gene Aliases: AW108311; AW123232; Pax; PXN

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UniProt ID: (Human) P49023, (Mouse) Q8VI36

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Entrez Gene ID: (Human) 5829, (Mouse) 19303

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Function(s)

integrin binding protein binding beta-catenin binding zinc ion binding vinculin binding protein kinase binding receptor signaling complex scaffold activity protein complex binding metal ion binding BH4 domain binding actin or actin-binding cytoskeletal protein

Process(es)

muscle contraction cell adhesion cell-matrix adhesion signal transduction signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway cellular response to reactive oxygen species vascular endothelial growth factor receptor signaling pathway growth hormone receptor signaling pathway activation of MAPK activity movement of cell or subcellular component cytoskeleton organization integrin-mediated signaling pathway regulation of cell shape peptidyl-tyrosine phosphorylation lamellipodium assembly substrate adhesion-dependent cell spreading positive regulation of protein kinase activity focal adhesion assembly branching morphogenesis of an epithelial tube

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Paxillin Polyclonal Antibody

Paxillin Antibody (10029-1-IG) in ICC/IF

Product Details

10029-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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