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Invitrogen
Perforin Polyclonal Antibody
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Product Details
PA5-87351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Amount
103 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse thymus, Mouse kidney; Cellular Location: Cell membrane, Cytoplasmic granule lumen, Endosome lumen, Multi-pass membrane protein
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytolysin; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; PRF1 (pore forming protein 1); RP11-710A11.3; unnamed protein product
Gene Aliases: Cyta; HPLH2; P1; Pfn; PFP; Prf-1; PRF1; RATCYTA
UniProt ID: (Human) P14222, (Mouse) P10820
Entrez Gene ID: (Human) 5551, (Mouse) 18646, (Rat) 50669
immunological synapse formation
plasma membrane repair
leukocyte mediated cytotoxicity
T cell mediated cytotoxicity
defense response to tumor cell
immune response to tumor cell
positive regulation of immune response to tumor cell
apoptotic process
cellular defense response
protein secretion
protein import
killing of cells of other organism
ceramide biosynthetic process
protein homooligomerization
protein maturation
defense response to virus
positive regulation of killing of cells of other organism
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
pyroptotic cell death
circadian rhythm
cytolysis
transmembrane transport
response to ethanol
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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