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          • Primary Antibodies ›
          • HRP Antibodies

          Rockland

          Peroxidase Polyclonal Antibody

          View all (16) HRP antibodies

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          Cite Peroxidase Polyclonal Antibody

          Product Details

          200-2138-0100

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunohistochemistry (IHC)

          1:1,000-1:5,000
          -

          ELISA (ELISA)

          1:20,000-1:100,000
          -

          Immunoprecipitation (IP)

          1:2,000-1:10,000
          -
          Product Specifications

          Species Reactivity

          Chemical

          Host/Isotype

          Guinea pig

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peroxidase [Horseradish]

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Ion-exchange chromatography

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

          Target Information

          HRP, also known as horseradish peroxidase, is an enzyme that is commonly used in biochemistry and molecular biology research. It is derived from the root of the horseradish plant and catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays, such as ELISAs and Western blots, due to its high sensitivity and stability. It can also be used as a tracer in enzyme-linked immunosorbent assays (ELISAs) and as a labeling agent in fluorescent microscopy.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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