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Invitrogen
Phospho-A-Raf (Tyr302) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-A-Raf (Tyr302) Antibody (PA5-36783) in WB
Western blot analysis of Phospho-A-Raf (Tyr302) in Lane 1: HeLa cell lysate treated with PMA (100 nM,15min), Lane 2: sp2/0 cell lysate treated with PMA (100 nM,15min), Lane 3: PC12 cell lysate treated with PMA (100 nM,15min). Samples were incubated with Phospho-A-Raf (Tyr302) polyclonal antibody (Product # PA5-36783) at a dilution of 1:500.
Product Details
PA5-36783
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human A-Raf around the phosphorylation site of Tyrosine 302
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 68 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Oncogene ARAF1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; RP1-230G1.1; Serine/threonine-protein kinase A-Raf; unnamed protein product; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
Gene Aliases: 1200013E08Rik; A-RAF; ARAF; ARAF1; AW495444; PKS; PKS2; RAFA1
UniProt ID: (Human) P10398, (Rat) P14056, (Mouse) P04627
Entrez Gene ID: (Human) 369, (Rat) 64363, (Mouse) 11836
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
receptor signaling protein activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
signal transduction
regulation of TOR signaling
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-serine phosphorylation
protein modification process
negative regulation of apoptotic process
activation of MAPKK activity
protein phosphorylation
intracellular signal transduction
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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