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Invitrogen

Phospho-AGO2 (Ser387) Polyclonal Antibody

View all (27) AGO2 antibodies

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Product Details

PA5-143647

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Ago2 (Ser-387) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 387 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. The site is also conserved in Ago1 and Ago4, but the homology surrounding the site is low between Ago2 and these two family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942875

Product Specific Information

This antibody was cross-absorbed to unphospho-Ago2 (Ser-387), before affinity purification using phospho-Ago2 (Ser-387) peptide (without carrier). The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A and mouse recombinant Ago2 full length protein. This reactivity is not observed after lambda phosphatase dephosphorylation.

Target Information

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AGO1; argonaute 2; argonaute 2, RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}; cancer susceptibility candidate 7 (non-protein coding); eIF-2C 1; eIF-2C 2; eIF-2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eIF2C; eIF2C 1; eIF2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eukaryotic translation initiation factor 2C, 2; GERp95; golgi ER protein 95 kDa; hAgo1; long intergenic non-protein coding RNA 980; mAgo2; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer

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Gene Aliases: 1110029L17Rik; 2310051F07Rik; AGO2; AI225898; AL022874; AW546247; CASC7; EIF2C2; ENSMUSG00000072493; Gerp95; Gm10365; Kiaa4215; LESKRES; LINC00980; mKIAA4215; PPD; Q10

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UniProt ID: (Human) Q9UKV8, (Mouse) Q8CJG0

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Entrez Gene ID: (Human) 27161, (Rat) 59117, (Mouse) 239528

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Function(s)
RNA 7-methylguanosine cap binding RNA polymerase II core binding core promoter sequence-specific DNA binding nucleic acid binding RNA binding double-stranded RNA binding single-stranded RNA binding mRNA binding translation initiation factor activity nuclease activity endonuclease activity endoribonuclease activity protein binding hydrolase activity endoribonuclease activity, producing 5'-phosphomonoesters siRNA binding miRNA binding mRNA 3'-UTR AU-rich region binding metal ion binding endoribonuclease activity, cleaving siRNA-paired mRNA endoribonuclease activity, cleaving miRNA-paired mRNA mRNA cap binding molecular_function poly(A) RNA binding pre-miRNA binding core promoter binding translation initiation factor
Process(es)
regulation of transcription, DNA-templated translation translational initiation regulation of translation post-embryonic development gene expression miRNA metabolic process positive regulation of gene expression production of siRNA involved in RNA interference gene silencing by RNA pre-miRNA processing cytoplasmic mRNA processing body assembly posttranscriptional gene silencing by RNA production of miRNAs involved in gene silencing by miRNA miRNA mediated inhibition of translation mRNA cleavage involved in gene silencing by miRNA negative regulation of amyloid precursor protein biosynthetic process RNA stabilization positive regulation of translation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation positive regulation of nuclear-transcribed mRNA poly(A) tail shortening small RNA loading onto RISC regulation of synapse maturation mRNA cleavage involved in gene silencing by siRNA positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay positive regulation of trophoblast cell migration transcription, DNA-templated mRNA cleavage biological_process cell differentiation miRNA loading onto RISC involved in gene silencing by miRNA RNA phosphodiester bond hydrolysis, endonucleolytic multicellular organism development RNA secondary structure unwinding siRNA loading onto RISC involved in RNA interference
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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