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Phospho-ATR (Ser428) Polyclonal Antibody

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Phospho-ATR (Ser428) Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis HeLa cells were fixed and permeabilized for detection of ATR (pS428) using ATR (pS428) Rabbit Polyclonal antibody (Product # 720107, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of ATR (pS428) (green) in the nucleus can be clearly observed in cells treated with hydroxyurea (HU, 3 mM, 20 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the ATR (pS428) peptide, which results in complete loss of signal. No competition was observed with the non-phospho peptide.

Phospho-ATR (Ser428) Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using Phospho-ATR pSer428 Antibody, Rabbit Polyclonal (Product # 720107), shows induction of Phospho-ATR pSer428 expression in the nucleus of HeLa cells upon treatment with Hydroxyurea. {TM}

Product Details

720107

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	2 µg/mL	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide corresponding to Human ATR (aa 424-432) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2607920

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Cat, Sheep and Bovine.

Target Information

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cyclin F is involved in response to cisplatin treatment in melanoma cell lines.

Oncology reports

Krajewski A,Gagat M,Żuryń A,Hałas-Wiśniewska M,Grzanka D,Grzanka A

Published figure using Phospho-ATR (Ser428) polyclonal antibody (Product # 720107) in Immunocytochemistry

Sun Mar 01 00:00:00 EST 2020

The ATM and Rad3-Related (ATR) Protein Kinase Pathway Is Activated by Herpes Simplex Virus 1 and Required for Efficient Viral Replication.

Journal of virology

Edwards TG,Bloom DC,Fisher C

720107 was used in Immunocytochemistry to study the phosphorylation and activation of the ATM and Rad3-related protein kinase pathway following Herpes Simplex Virus 1 infection.

Thu Mar 15 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8

Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1

UniProt ID: (Human) Q13535

Entrez Gene ID: (Human) 545

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

Function(s)

DNA damage checkpoint DNA replication DNA repair cellular response to DNA damage stimulus cell cycle multicellular organism development negative regulation of DNA replication peptidyl-serine phosphorylation positive regulation of telomere maintenance via telomerase cellular response to UV interstrand cross-link repair response to drug positive regulation of DNA damage response, signal transduction by p53 class mediator protein autophosphorylation protein localization to chromosome, telomeric region cellular response to gamma radiation replicative senescence establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere regulation of cellular response to heat regulation of signal transduction by p53 class mediator positive regulation of telomerase catalytic core complex assembly

Process(es)

DNA binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding MutLalpha complex binding MutSalpha complex binding

Phospho-ATR (Ser428) Polyclonal Antibody

Product Details

720107

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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