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Phospho-B-Raf (Ser446) Polyclonal Antibody

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Phospho-B-Raf (Ser446) Polyclonal Antibody

Product Details

BS-5232R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Ser446.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; BRAF-1; Braf-2; BRAF1; Braf2; C230098H17; C87398; D6Ertd631e; NS7; RAFB1

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UniProt ID: (Human) P15056, (Mouse) P28028

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding identical protein binding metal ion binding scaffold protein binding protein serine kinase activity mitogen-activated protein kinase kinase binding protein heterodimerization activity receptor signaling protein activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade myeloid progenitor cell differentiation protein phosphorylation signal transduction epidermal growth factor receptor signaling pathway visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration positive regulation of glucose transport synaptic vesicle exocytosis cell differentiation thyroid gland development T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading somatic stem cell population maintenance regulation of cell proliferation negative regulation of apoptotic process stress fiber assembly CD4-positive, alpha-beta T cell differentiation positive T cell selection CD4-positive or CD8-positive, alpha-beta T cell lineage commitment negative regulation of neuron apoptotic process regulation of T cell differentiation alpha-beta T cell differentiation thymus development regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly long-term synaptic potentiation head morphogenesis face development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cellular response to xenobiotic stimulus endothelial cell apoptotic process establishment of protein localization to membrane postsynaptic modulation of chemical synaptic transmission positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process activation of MAPKK activity cellular response to drug response to peptide hormone protein heterooligomerization response to cAMP glucose transport phosphorylation intracellular signal transduction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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