Invitrogen

Phospho-Histone H3 (Ser10) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

21 References

View all (184) Histone H3 antibodies

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Phospho-Histone H3 (Ser10) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-Histone H3 (ser 10) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Histone H3 (Ser10) Rabbit Polyclonal antibody (Product # PA5-17869) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization in dividing cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Phospho-Histone H3 (Ser10) Antibody in Western Blot (WB) — Western blot analysis was performed on acid extracts (20 µg lysate) of HL-60 (Lane 1), HL-60 treated with Calyculin A (50 ng/mL for 30 minutes) (Lane 2), NIH/3T3 (Lane 3) and NIH/3T3 treated with Nocodazole (100 ng/mL for 18 hours) (Lane 4). The blot was probed with Anti-Phospho-Histone H3 (Ser10) (Product # PA5-17869, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Phospho-Histone H3 (Ser10) was observed across the cell lines tested and enhanced upon treatment.

Phospho-Histone H3 (Ser10) Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serµm/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serµm/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.

Phospho-Histone H3 (Ser10) Antibody in Peptide array (Array) — The specificity of the antibody, Anti-Phospho-Histone H3 (Ser10) Polyclonal Antibody (Product # PA5-17869, 1:2000 dilution) to Histone H3pS10 peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel).

Phospho-Histone H3 (Ser10) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — 5 Figure Progesterone promotes CM proliferation through up-regulation of YAP expression. Cultured P7 CMs were treated with control (vehicle, DMSO) or progesterone (10 -7 M), alone or in combination with RU486 (A-C) or YAP siRNAs (D-F). A, Proliferation regulator mRNA expressions of CM were analysed by quantitative PCR (n = 4). B, Representative immunoblots (B1) and quantification (B2) of YAP protein expression were analysed by Western blotting (GAPDH was used as internal control) (n = 4). C, The mRNA expression of YAP target genes ANKRD1, CYR61 and CTGF was analysed by quantitative real-time PCR (n = 4). D-F, CM proliferation was evaluated by Ki67, PH3 and Aurora B immunostaining. Representative images are shown in D1, E1 and F1, and quantification of percentages of Ki67 + , PH3 + or Aurora B + CMs is shown in D2, E2 and F2, respectively (3000-5000 cells were randomly selected and analysed for each group) (n = 4). Scale bars are 40 um ( ** P < .01, *** P < .001) vs Control; ( ## P < .01, ### P < .001) vs Progesterone. CM, cardiomyocyte; YAP, yes-associated protein

Phospho-Histone H3 (Ser10) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 1. CAF1 cells are mitotically active. (A) Identification of human primary ovarian NFs and CAFs. Immunostaining of FSP1 and western blot analysis of alpha-SMA in NFs and CAFs. Cells were stained with FSP1 (green) and counterstained with DAPI (blue). The relative intensity of alpha-SMA normalized to housekeeping protein was shown at the bottom. (B) Cell cycle analysis of NFs and CAFs. (C) The cell cycle phase distribution in each cell line (as in B). (D) Pathways enriched in CAF1 was revealed by GSEA indicated that CAF1 cells were mitotically active. (E) Immunostaining of phospho-histone H3 in NF1 and CAF1 cells. Cells were stained with phospho-histone H3 (green) and counterstained with DAPI (blue). (F) Western blot analysis of phospho-histone H3 in NF1 and CAF1 cells. The relative intensity of phospho-histone H3 normalized to housekeeping protein was shown at the bottom.

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (IHC) — Figure 2. Evidence of SARS-CoV-2 in human FP. Panel A shows tongue photographs of patient #114 moments prior to biopsy of her FP during the course of COVID-19 and 3 months later. Panel B shows H&E staining of a section through the FP from patient #114 that contained two taste buds; the consecutive section was used for ISH outlining the presence of viral particles (SARS-CoV-2 S ) probe for the Spike mRNA and the SARS-CoV-2 ( ORF1ab ) probe for the replicating virus, shown in Figure 2C. Panel C, an antisense probe specific to the genomic positive strand RNA of the spike protein ( S ) sequence of SARS-CoV-2 and a sense probe to the SARS-CoV-2 ORF1ab negative strand RNA indicate the presence of replicating virus in PLCB2 positive cells (participant #114). Note the arrow pointing to another viral positive cell in the neighboring taste bud. Panel D demonstrates the proliferation of the stem cell layer of the FP by immunostaining for the marker of all active phases of cell cycle Ki67 and the late G2 and M phase markers phosphorylated histone H3 (PHH3). Top left-sided images show a FP from an age- and sex-matched control participant for #114 compared with during and post SARS-CoV-2 infection. White arrows indicate the breaks in the otherwise continuous layer of stem cells. Right-sided panels show a continuous stem cell layer in participant #089 pre, but multiple breaks especially at 6 weeks, and less at 10 weeks, post SARS-CoV-2. Panel E shows the percentages of total cells (as deter

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA)) — Figure 1 Serum progesterone concentration declines after birth and is associated with decrease in CM proliferation. A,B, Evaluation of CM proliferation by Ki67 and PH3 immunostaining at E16, P1, P7 and P14 hearts. Representative images with z-stacking are shown in A1 and B1, and quantification of percentages of Ki67 + and PH3 + CMs is shown in A2 and B2, respectively (n = 5). Scale bars are 20 um. cTnT indicates cardiac troponin T. C, Serum concentrations of progesterone in mice at E16, P1, P7 and P14 detected using ELISA kit (n = 5-8). *** P < .001 vs E16; ### P < .001 vs P1; &&& P < .001 vs P7. CM, cardiomyocyte

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — 2 Figure Progesterone supplementation promotes postnatal CM proliferation in vivo. A, Overview of the experimental set-up of the daily intraperitoneal injection of progesterone (8 mg/kg) or control vehicle (corn oil) from P1 to P6. Hearts were harvested at P7. B-D, CM proliferation was evaluated by Ki67, PH3 and Aurora B immunostaining. Representative images with z-stacking are shown in B1, C1 and D1, and quantification of percentages of Ki67 + , PH3 + or Aurora B + CMs is shown in B2, C2 and D2, respectively (n = 6). Scale bars are 20 um. E, Representative images (E1) and quantification (E2) of the cell size of CMs (labelled by cTnT and pan-cadherin to show intact cardiomyocytes) isolated from P7 heart. Eighty to one hundred cells were randomly selected per heart (n = 6). Scale bars are 20 um. F, Heart weight and body weight (HW/BW) ratio (n = 10-11). G, The mRNA expression of cell cycle activators in mouse hearts was analysed by real-time PCR (n = 5). CM, cardiomyocyte

Phospho-Histone H3 (Ser10) Antibody — Detection of differential subcellular localization of the target protein demonstrates antibody specificity. Immunofluorescence analysis of Phospho-Histone H3(Ser10) using anti-Phospho-Histone H3(Ser10) Polyclonal antibody (Product # PA5-17869 ) shows localization of Phospho-Histone H3 (Ser 10) predominantly in nucleus of dividing HeLa cells. {RE}

Product Details

PA5-17869

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:400	-
Immunocytochemistry (ICC/IF)	1:800	View 5 publications 5 publications
Flow Cytometry (Flow)	1:50	View 1 publication 1 publication
Peptide Array (Array)	1:2,000	-
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Fruit fly, Human, Mouse, Non-human primate, Rat, Xenopus
Published species	C. elegans, Fruit fly, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3
Conjugate	Unconjugated
Form	Liquid
Concentration	Lot specific, see label
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10984484

Product Specific Information

Phospho-Histone H3 (Ser10) Antibody detects endogenous levels of histone H3 only when phosphorylated at Ser10; however, this antibody does not detect phosphorylated Ser10 when Lys9 is acetylated or methylated. This antibody does not cross-react with histone H3 phosphorylated at Ser28.

This antibody is not cross-reactive with other phosphorylated histones or with acetylated histones.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA Signatures of the Developing Primate Fovea.

Frontiers in cell and developmental biology

Fishman ES,Louie M,Miltner AM,Cheema SK,Wong J,Schlaeger NM,Moshiri A,Simó S,Tarantal AF,La Torre A

Published figure using Phospho-Histone H3 (Ser10) polyclonal antibody (Product # PA5-17869) in Immunohistochemistry

Sat Jul 16 00:00:00 EDT 2022

Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death.

Cell death & disease

Saha S,Verma R,Kumar C,Kumar B,Dey AK,Surjit M,Mylavarapu SVS,Maiti TK

PA5-17869 was used in Flow cytometry/Cell sorting to provide evidence of PA-induced hepatocellular death mediated by deubiquitinase USP7 downregulation and subsequent mitotic catastrophe.

Wed Jun 22 00:00:00 EDT 2022

The exocyst complex regulates C. elegans germline stem cell proliferation by controlling membrane Notch levels.

Development (Cambridge, England)

Pushpa K,Dagar S,Kumar H,Pathak D,Mylavarapu SVS

PA5-17869 was used in Immunohistochemistry to reveal that the exocyst complex regulates C. elegans GSC proliferation by modulating Notch signaling cell autonomously.

Sun Aug 01 00:00:00 EDT 2021

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

PA5-17869 was used in Immunocytochemistry to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 EDT 2021

Human Taste Cells Express ACE2: a Portal for SARS-CoV-2 Infection.

bioRxiv : the preprint server for biology

Doyle ME,Appleton A,Liu QR,Yao Q,Mazucanti CH,Egan JM

PA5-17869 was used in Immunohistochemistry to investigate whether ACE2 is expressed on taste receptor cells (TRCs) nor if TRCs are infected directly.

Wed Apr 21 00:00:00 EDT 2021

Human Taste Cells Express ACE2: a Portal for SARS-CoV-2 Infection.

bioRxiv : the preprint server for biology

Doyle ME,Appleton A,Liu QR,Yao Q,Mazucanti CH,Egan JM

PA5-17869 was used in Immunohistochemistry to investigate whether ACE2 is expressed on taste receptor cells (TRCs) nor if TRCs are infected directly.

Wed Apr 21 00:00:00 EDT 2021

Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis.

PLoS genetics

Pizzo L,Lasser M,Yusuff T,Jensen M,Ingraham P,Huber E,Singh MD,Monahan C,Iyer J,Desai I,Karthikeyan S,Gould DJ,Yennawar S,Weiner AT,Pounraja VK,Krishnan A,Rolls MM,Lowery LA,Girirajan S

PA5-17869 was used in Western Blot to suggest that several 16p12.1 genes sensitize the genome towards neurodevelopmental defects, and complex interactions with "second-hit" genes determine the ultimate phenotypic manifestation.

Thu Apr 01 00:00:00 EDT 2021

Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis.

PLoS genetics

Pizzo L,Lasser M,Yusuff T,Jensen M,Ingraham P,Huber E,Singh MD,Monahan C,Iyer J,Desai I,Karthikeyan S,Gould DJ,Yennawar S,Weiner AT,Pounraja VK,Krishnan A,Rolls MM,Lowery LA,Girirajan S

Thu Apr 01 00:00:00 EDT 2021

Mud Loss Restricts Yki-Dependent Hyperplasia in Drosophila Epithelia.

Journal of developmental biology

Parra AS,Johnston CA

PA5-17869 was used in Immunohistochemistry-immunofluorescence to suggest that ECM/BM remodeling can limit untoward growth initiated by an important driver of tumor growth and highlight a potential regulatory link with cytoskeleton-associated genes.

Sun Dec 13 00:00:00 EST 2020

Mud Loss Restricts Yki-Dependent Hyperplasia in Drosophila Epithelia.

Journal of developmental biology

Parra AS,Johnston CA

Sun Dec 13 00:00:00 EST 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

PA5-17869 was used in Immunohistochemistry to show that FZD5 is a novel target for the development of therapeutic strategies to overcome chemoresistance and prevent recurrence in TNBC.

Sat Dec 12 00:00:00 EST 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

PA5-17869 was used in Immunohistochemistry to show that FZD5 is a novel target for the development of therapeutic strategies to overcome chemoresistance and prevent recurrence in TNBC.

Sat Dec 12 00:00:00 EST 2020

Loss of FOXF1 expression promotes human lung-resident mesenchymal stromal cell migration via ATX/LPA/LPA1 signaling axis.

Scientific reports

Cao P,Walker NM,Braeuer RR,Mazzoni-Putman S,Aoki Y,Misumi K,Wheeler DS,Vittal R,Lama VN

PA5-17869 was used in Western Blotting to identify FOXF1 as a novel transcriptional repressor of ATX and demonstrate that loss of FOXF1 promotes LR-MSC migration via the ATX/LPA/LPA1 signalling axis.

Fri Dec 04 00:00:00 EST 2020

Loss of FOXF1 expression promotes human lung-resident mesenchymal stromal cell migration via ATX/LPA/LPA1 signaling axis.

Scientific reports

Cao P,Walker NM,Braeuer RR,Mazzoni-Putman S,Aoki Y,Misumi K,Wheeler DS,Vittal R,Lama VN

PA5-17869 was used in Western Blotting to identify FOXF1 as a novel transcriptional repressor of ATX and demonstrate that loss of FOXF1 promotes LR-MSC migration via the ATX/LPA/LPA1 signalling axis.

Fri Dec 04 00:00:00 EST 2020

Progesterone, via yes-associated protein, promotes cardiomyocyte proliferation and cardiac repair.

Cell proliferation

Lan C,Cao N,Chen C,Qu S,Fan C,Luo H,Zeng A,Yu C,Xue Y,Ren H,Li L,Wang H,Jose PA,Xu Z,Zeng C

PA5-17869 was used in Western Blotting to uncover a role of circulating progesterone withdrawal as a novel mechanism for the postnatal loss of mammalian cardiac regenerative potential.

Sun Nov 01 00:00:00 EDT 2020

Progesterone, via yes-associated protein, promotes cardiomyocyte proliferation and cardiac repair.

Cell proliferation

Lan C,Cao N,Chen C,Qu S,Fan C,Luo H,Zeng A,Yu C,Xue Y,Ren H,Li L,Wang H,Jose PA,Xu Z,Zeng C

PA5-17869 was used in Western Blotting to uncover a role of circulating progesterone withdrawal as a novel mechanism for the postnatal loss of mammalian cardiac regenerative potential.

Sun Nov 01 00:00:00 EDT 2020

Progesterone, via yes-associated protein, promotes cardiomyocyte proliferation and cardiac repair.

Cell proliferation

Lan C,Cao N,Chen C,Qu S,Fan C,Luo H,Zeng A,Yu C,Xue Y,Ren H,Li L,Wang H,Jose PA,Xu Z,Zeng C

PA5-17869 was used in Western Blotting to uncover a role of circulating progesterone withdrawal as a novel mechanism for the postnatal loss of mammalian cardiac regenerative potential.

Sun Nov 01 00:00:00 EDT 2020

Progesterone, via yes-associated protein, promotes cardiomyocyte proliferation and cardiac repair.

Cell proliferation

Lan C,Cao N,Chen C,Qu S,Fan C,Luo H,Zeng A,Yu C,Xue Y,Ren H,Li L,Wang H,Jose PA,Xu Z,Zeng C

PA5-17869 was used in Western Blotting to uncover a role of circulating progesterone withdrawal as a novel mechanism for the postnatal loss of mammalian cardiac regenerative potential.

Sun Nov 01 00:00:00 EDT 2020

Agrin Promotes Limbal Stem Cell Proliferation and Corneal Wound Healing Through Hippo-Yap Signaling Pathway.

Investigative ophthalmology & visual science

Hou L,Fu W,Liu Y,Wang Q,Wang L,Huang Y

PA5-17869 was used in Western Blotting to show how agrin promotes the proliferation of limbal stem cells and accelerates the healing rate of cornceal would through Hippo-Yap signalling pathway.

Mon May 11 00:00:00 EDT 2020

OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome.

Cell stem cell

Xu R,Brawner AT,Li S,Liu JJ,Kim H,Xue H,Pang ZP,Kim WY,Hart RP,Liu Y,Jiang P

PA5-17869 was used in Western Blotting to conclude that altered OLIG2 expression may underlie neurodevelopmental abnormalities and cognitive defects in DS patients.

Thu Jun 06 00:00:00 EDT 2019

Dynamics and Spatial Genomics of the Nascent Transcriptome by Intron seqFISH.

Cell

Shah S,Takei Y,Zhou W,Lubeck E,Yun J,Eng CL,Koulena N,Cronin C,Karp C,Liaw EJ,Amin M,Cai L

PA5-17869 was used in Immunocytochemistry-immunoflourescence to conclude that spatial genomics of the nascent transcriptome by intron seqFISH reveals nuclear organizational principles and fast dynamics in single cells that are otherwise obscured.

Thu Jul 12 00:00:00 EDT 2018

Aurora A kinase activity is required to maintain an active spindle assembly checkpoint during prometaphase.

Journal of cell science

Courtheoux T,Diallo A,Damodaran AP,Reboutier D,Watrin E,Prigent C

PA5-17869 was used in Western Blotting to report that inhibition of Aurora A leads to loss of the spindle assembly checkpoint even in the presence of nocodazole or Taxol.

Thu Apr 12 00:00:00 EDT 2018

A sleep state in Drosophila larvae required for neural stem cell proliferation.

eLife

Szuperak M,Churgin MA,Borja AJ,Raizen DM,Fang-Yen C,Kayser MS

PA5-17869 was used in Immunohistochemistry to identify a sleep state in Drosophila larvae as a critical factor for normal proliferation of neural stem cells.

Fri Feb 09 00:00:00 EST 2018

RAD52 is required for RNA-templated recombination repair in post-mitotic neurons.

The Journal of biological chemistry

Welty S,Teng Y,Liang Z,Zhao W,Sanders LH,Greenamyre JT,Rubio ME,Thathiah A,Kodali R,Wetzel R,Levine AS,Lan L

PA5-17869 was used in Immunocytochemistry-immunoflourescence to reveal a novel RNA-dependent repair mechanism of DSBs in post-mitotic neurons and defects in this pathway may contribute to diseases such as Alzheimer's.

Fri Jan 26 00:00:00 EST 2018

RAD52 is required for RNA-templated recombination repair in post-mitotic neurons.

The Journal of biological chemistry

Welty S,Teng Y,Liang Z,Zhao W,Sanders LH,Greenamyre JT,Rubio ME,Thathiah A,Kodali R,Wetzel R,Levine AS,Lan L

Fri Jan 26 00:00:00 EST 2018

Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress.

Cell cycle (Georgetown, Tex.)

Wang Q,Xue L,Zhang X,Bu S,Zhu X,Lai D

PA5-17869 was used in immunocytochemistry and western blot to investigate protection of ovarian cancer-associated fibroblasts against oxidative stress by autophagy

Wed May 18 00:00:00 EDT 2016

Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress.

Cell cycle (Georgetown, Tex.)

Wang Q,Xue L,Zhang X,Bu S,Zhu X,Lai D

PA5-17869 was used in immunocytochemistry and western blot to investigate protection of ovarian cancer-associated fibroblasts against oxidative stress by autophagy

Wed May 18 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: CG31613-PA; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; His3:CG31613-PA; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; Histone Cluster 2 H3a; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

Gene Aliases: CG31613; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; Dmel\CG31613; Dmel_CG31613; H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; h3r; His3; His3:CG31613; His3:CG33803; His3:CG33806; His3:CG33809; His3:CG33812; His3:CG33815; His3:CG33818; His3:CG33821; His3:CG33824; His3:CG33827; His3:CG33830; His3:CG33833; His3:CG33836; His3:CG33839; His3:CG33842; His3:CG33845; His3:CG33848; His3:CG33851; His3:CG33854; His3:CG33857; His3:CG33860; His3:CG33863; His3:CG33866; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; XELAEV_18028537mg

UniProt ID: (Human) P68431, (Human) Q6NWP8, (Human) Q71DI3, (Fruit fly) P02299, (Mouse) P68433, (Mouse) P84228

Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Fruit fly) 318847, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Xenopus) 399088

Molecular Function: histone

Function(s)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

Process(es)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion nucleosomal DNA binding

Phospho-Histone H3 (Ser10) Polyclonal Antibody

Product Details

PA5-17869

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Peptide Array (Array)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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