Invitrogen

Phospho-Lyn (Tyr507) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details

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Phospho-Lyn (Tyr507) Antibody in Immunofluorescence (IF) — Immunofluorescence was performed on fixed and permeabilized Jurkat cells treated with Pervanadate (100 uMl/ 60 min) for detection of Lyn (pY507) using Anti-Lyn (pY507)Rabbit Polyclonal Antibody (Product # 720013, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Lyn (pY507) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Lyn (pY507). Panel e) represents control cells with no primary antibody to assess background.

Phospho-Lyn (Tyr507) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (20 µg lysate) of Jurkat (lane1), Jurkat treated with Pervanadate (100 uM/ 60min) (lane2), K562 (lane3) and K562 treated with Pervanadate (100 uM/ 60min) (lane4). The blots were probed with Anti-Lyn (pY507)Rabbit Polyclonal Antibody (Product # 720013, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 45 kDa band corresponding to Lyn (pY507) was observed across cell lines tested according to the treatment. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Phospho-Lyn (Tyr507) Antibody in Cell Treatment — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Phospho-Lyn (Tyr507) Polyclonal Antibody (Product # 720013), shows increased expression of proteins phosphorylated at the tyrosine residues in Jurkat and K562 cell lines upon treatment with Pervanadate. {TM}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Western Blot (WB)

0.5-2 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phosphopeptide against Human Lyn (aa 503-512)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532787

Product Specific Information

This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog.

Target Information

Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Lck/Yes-related novel protein tyrosine kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog

Gene Aliases: JTK8; LYN; p53Lyn; p56Lyn

UniProt ID: (Human) P07948

Entrez Gene ID: (Human) 4067

Molecular Function: kinase non-receptor tyrosine protein kinase protein kinase transferase

B cell homeostasis regulation of cytokine production regulation of protein phosphorylation negative regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway adaptive immune response Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus response to sterol depletion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway central nervous system development blood coagulation positive regulation of cell proliferation negative regulation of cell proliferation response to toxic substance response to hormone response to carbohydrate positive regulation of neuron projection development oligodendrocyte development response to organic cyclic compound viral process peptidyl-tyrosine phosphorylation signal transduction by protein phosphorylation platelet activation erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development T cell costimulation lipopolysaccharide-mediated signaling pathway cellular response to extracellular stimulus response to insulin regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway cellular response to heat peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to drug positive regulation of tyrosine phosphorylation of STAT protein response to amino acid regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of erythrocyte differentiation protein autophosphorylation ephrin receptor signaling pathway response to axon injury cytokine secretion regulation of cytokine secretion regulation of inflammatory response negative regulation of immune response B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway leukocyte migration positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process

Process(es)

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding