Invitrogen

Phospho-PKC alpha (Ser657) Polyclonal Antibody

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Phospho-PKC alpha (Ser657) Polyclonal Antibody

Product Details

PA5-78124

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser657 of human PKC alpha. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736408

Product Specific Information

Positive Control: HeLa

Predicted Reactivity: Mouse (100%), Rat (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C, alpha

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Gene Aliases: AAG6; PKC-alpha; PKCA; PRKACA; PRKCA

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UniProt ID: (Human) P17252

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Entrez Gene ID: (Human) 5578, (Rat) 24680

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) non-receptor serine/threonine protein kinase
Process(es)
angiogenesis positive regulation of endothelial cell proliferation desmosome assembly protein phosphorylation mitotic nuclear envelope disassembly cell adhesion activation of adenylate cyclase activity negative regulation of adenylate cyclase activity positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy peptidyl-serine phosphorylation platelet activation positive regulation of cell migration positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of glial cell apoptotic process histone H3-T6 phosphorylation intracellular signal transduction regulation of mRNA stability positive regulation of blood vessel endothelial cell migration positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of mitotic cell cycle regulation of insulin secretion positive regulation of ERK1 and ERK2 cascade response to interleukin-1 regulation of platelet aggregation apoptotic signaling pathway positive regulation of dense core granule biogenesis inactivation of MAPK activity response to reactive oxygen species negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of the force of heart contraction chondrocyte differentiation negative regulation of protein kinase activity cellular calcium ion homeostasis apoptotic process regulation of muscle contraction aging learning or memory negative regulation of cell proliferation response to mechanical stimulus response to toxic substance response to organic cyclic compound negative regulation of translation peptidyl-threonine phosphorylation central nervous system neuron axonogenesis neutrophil chemotaxis response to estradiol peptidyl-serine autophosphorylation response to peptide hormone establishment of protein localization response to ethanol negative regulation of heart contraction positive regulation of exocytosis negative regulation of glucose import negative regulation of insulin receptor signaling pathway response to antibiotic protein autophosphorylation regulation of receptor-mediated endocytosis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis response to corticosterone positive regulation of synapse assembly cellular response to carbohydrate stimulus intrinsic apoptotic signaling pathway
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