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          • Primary Antibodies ›
          • RAC1/CDC42 Antibodies

          Invitrogen

          Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody

          Advanced Verification
          1 Published Figure
          1 Reference
          View all (7) RAC1/CDC42 antibodies

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          Cite Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (1)
          • Advanced Verification (1)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-RAC1/CDC42 (Ser71) Antibody (44-214G) in IHC (P)

          Immunohistochemistry analysis of Rac1/cdc42 (pS71) showing staining in the cytoplasm of paraffin-embedded human stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room te... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-RAC1/CDC42 (Ser71) Antibody in Western Blot (WB)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-RAC1/CDC42 (Ser71) Antibody
          Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody

          Product Details

          44-214G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:100
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rac1-protein that contains serine 71. The sequence is conserved in human and mouse Rac 1, 2, and 3, and cdc42 human, mouse, rat, dog and frog.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533607

          Target Information

          Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cell division control protein 42 homolog; Cell migration-inducing gene 5 protein; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein G25K; p21-Rac1; Rac1ins; ras-like protein; Ras-like protein TC25; ras-related C3 botulinum toxin substrate; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); small GTP binding protein; small GTP binding protein CDC42; unnamed protein product

          View more View less

          Gene Aliases: CDC42; CDC42Hs; G25K; MIG5; MRD48; p21-Rac1; Rac-1; RAC1; TC-25; TC25; TKS

          View more View less

          UniProt ID: (Human) P60953, (Human) P63000

          View more View less

          Entrez Gene ID: (Human) 998, (Human) 5879

          View more View less

          Function(s)
          nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity protein kinase binding GTP-dependent protein binding thioesterase binding GBD domain binding apolipoprotein A-I receptor binding identical protein binding ubiquitin protein ligase activity enzyme binding macromolecular complex binding Rho GDP-dissociation inhibitor binding
          Process(es)
          heart process cardiac conduction system development cardiac neural crest cell migration involved in outflow tract morphogenesis endocytosis phagocytosis, engulfment actin filament organization Golgi organization regulation of mitotic nuclear division nuclear migration establishment or maintenance of cell polarity signal transduction integrin-mediated signaling pathway small GTPase mediated signal transduction nervous system development protein localization anatomical structure morphogenesis regulation of lamellipodium assembly positive regulation of lamellipodium assembly substantia nigra development establishment of cell polarity actin cytoskeleton organization cell differentiation macrophage differentiation positive regulation of cell growth positive regulation of cell migration positive regulation of pseudopodium assembly negative regulation of protein complex assembly positive regulation of cytokinesis regulation of actin cytoskeleton organization cell junction assembly adherens junction organization embryonic heart tube development establishment or maintenance of apical/basal cell polarity dendritic cell migration neuropilin signaling pathway positive regulation of MAPK cascade modulation by host of viral process establishment of epithelial cell apical/basal polarity filopodium assembly positive regulation of pinocytosis neuron fate determination positive regulation of cellular component organization regulation of filopodium assembly positive regulation of filopodium assembly regulation of stress fiber assembly positive regulation of stress fiber assembly nucleus localization establishment of localization in cell establishment of Golgi localization positive regulation of protein kinase B signaling regulation of attachment of spindle microtubules to kinetochore heart contraction Wnt signaling pathway, planar cell polarity pathway positive regulation of epithelial cell proliferation involved in lung morphogenesis dendritic spine morphogenesis cellular response to interferon-gamma organelle transport along microtubule endothelin receptor signaling pathway involved in heart process regulation of postsynapse organization positive regulation of substrate adhesion-dependent cell spreading neuron migration positive regulation of protein phosphorylation auditory receptor cell morphogenesis sphingosine-1-phosphate signaling pathway epithelial cell morphogenesis inflammatory response hyperosmotic response cytoskeleton organization cell adhesion cell-matrix adhesion enzyme linked receptor protein signaling pathway G-protein coupled receptor signaling pathway axon guidance motor neuron axon guidance regulation of cell shape response to wounding regulation of hydrogen peroxide metabolic process positive regulation of endothelial cell migration regulation of fibroblast migration negative regulation of fibroblast migration regulation of cell-substrate adhesion positive regulation of cell-substrate adhesion regulation of neuron maturation dendrite development cell migration Rac protein signal transduction cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development regulation of cell morphogenesis cell projection assembly lamellipodium assembly actin filament polymerization regulation of cell migration positive regulation of actin filament polymerization cortical cytoskeleton organization forebrain development positive regulation of microtubule polymerization ruffle organization response to lipopolysaccharide negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction non-canonical Wnt signaling pathway positive regulation of insulin secretion involved in cellular response to glucose stimulus superoxide anion generation erythrocyte enucleation engulfment of apoptotic cell cell-cell junction organization regulation of nitric oxide biosynthetic process respiratory burst regulation of JAK-STAT cascade hepatocyte growth factor receptor signaling pathway regulation of neuronal synaptic plasticity negative regulation of receptor-mediated endocytosis anatomical structure arrangement neuron projection morphogenesis dendrite morphogenesis cell motility homeostasis of number of cells within a tissue localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic regulation of respiratory burst cell chemotaxis positive regulation of dendritic spine development regulation of cell adhesion involved in heart morphogenesis regulation of ERK5 cascade semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane angiotensin-activated signaling pathway involved in heart process positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly regulation of postsynapse assembly positive regulation of ruffle assembly regulation of synaptic vesicle endocytosis regulation of neutrophil migration positive regulation of bicellular tight junction assembly positive regulation of skeletal muscle acetylcholine-gated channel clustering interneuron migration midbrain dopaminergic neuron differentiation positive regulation of ovarian follicle development regulation of neuron migration
          It has to be done as per old AB suggested Products section.

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