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Phospho-RPA2 (Ser33) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-RPA2 (Ser33) Antibody (PA5-39809) in WB
Western blot analysis of phosphor-RPA2 (S33) was performed by loading whole cell lysate in 1X SDS sample buffer with 2-ME from 2 x 105 mouse embryonic fibroblast cells treated with (+) or without (-) 2mM hydroxyurea (HU) for 1.5 hours. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # WG1402BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-39809
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphopeptide derived from human RFA2 around the phosphorylation site of Ser33 (A-P-SP-Q-A)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-39809 has been used successfully in peptide-ELISA at a dilution of 1:10,000.
Target Information
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 30-kDa protein; HSSB; p32; p32-subunit of replication protein A; p34; replication factor A protein 2; replication protein A; Replication protein A 32kDa subunit; replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RPA2; RpLP1; RpP2; unnamed protein product
Gene Aliases: REPA2; Rf-A2; RP-A p32; RP-A p34; RPA32; RPA34
UniProt ID: (Human) P15927, (Mouse) Q62193, (Rat) Q63528
Entrez Gene ID: (Human) 6118, (Mouse) 19891, (Rat) 59102
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
DNA repair
base-excision repair
nucleotide-excision repair
mismatch repair
DNA recombination
regulation of double-strand break repair via homologous recombination
mitotic G1 DNA damage checkpoint
protein localization to chromosome
regulation of DNA damage checkpoint
DNA damage checkpoint
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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