Invitrogen

Phospho-RSK1 (Thr573) Polyclonal Antibody

Product Image Gallery

Phospho-RSK1 (Thr573) Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Phospho-p90RSK pThr573 in HeLa cells, TPA-treated, using a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Phospho-RSK1 (Thr573) Antibody in Western Blot (WB) — Western blot analysis of Phospho-p90RSK (pThr573) was performed by loading 20 µg of HEK293T whole cell lysates, from cells either left untreated (Lane 1) or induced with 100 nM PMA (Lane 2) for 15 minutes, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) at a dilution of 1:250 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:400

Immunofluorescence (IF)

1:400

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Chicken, Hamster, Human, Mouse, Rat, Xenopus, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr573 of human p90RSK

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10989467

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1; MAPK-activated protein kinase 1a; MAPKAP kinase 1; MAPKAP kinase 1a; MAPKAPK-1; MAPKAPK-1a; MAPKAPK1A; OTTHUMP00000004114; P90-RSK; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 2 alpha; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase II alpha; ribosomal protein S6 kinase II beta; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1 L homeolog; Ribosomal S6 kinase 1; RSK-1; S6 kinase II beta; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1; S6KII-alpha; S6KII-beta

Gene Aliases: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1; wu:fc56h07; XELAEV_18012109mg; zgc:152654

UniProt ID: (Chicken) P18652, (Human) Q15418, (Rat) Q63531, (Mouse) P18653

Entrez Gene ID: (Chicken) 386579, (Human) 6195, (Chinese hamster) 100770849, (Rat) 81771, (Mouse) 20111, (Zebrafish) 777728, (Xenopus) 445821

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase