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Invitrogen

Phospho-RUNX2 (Ser28) Polyclonal Antibody

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View all (39) RUNX2 antibodies

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Phospho-RUNX2 (Ser28) Polyclonal Antibody

Product Details

PA5-105643

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

-
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RUNX2(Accession Q13950), corresponding to amino acid residues around phosphorylated Ser28.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817071

Product Specific Information

Antibody detects endogenous levels of RUNX2 only when phosphorylated at S28.

Target Information

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acute myeloid leukemia 3 protein; AKV core binding factor; AML-3; core binding factor alpha 1; core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; nuclear protein; oncogene AML-3; osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2 alpha A; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; transcription factor; transcription factor Cbfa1/Osf2

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Gene Aliases: AML3; Cbf; CBF-alpha-1; Cbfa-1; CBFA1; CCD; CCD1; CLCD; LS3; OSF-2; OSF2; PEA2aA; Pebp2a1; PEBP2aA; Pebpa2a

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UniProt ID: (Human) Q13950, (Mouse) Q08775

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Entrez Gene ID: (Human) 860, (Mouse) 12393, (Rat) 367218

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding sequence-specific double-stranded DNA binding transcription regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding protein domain specific binding chromatin DNA binding histone deacetylase binding bHLH transcription factor binding general transcription initiation factor binding DNA-binding transcription factor binding
Process(es)
skeletal system development ossification osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte differentiation chondrocyte development osteoblast development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transmembrane receptor protein serine/threonine kinase signaling pathway cell proliferation positive regulation of cell proliferation gene expression regulation of gene expression positive regulation of Schwann cell proliferation positive regulation of gene expression hemopoiesis neuron differentiation T cell differentiation regulation of ossification BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis ligamentous ossification regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth regulation of cell differentiation regulation of osteoblast differentiation positive regulation of osteoblast differentiation positive regulation of ossification negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development cell maturation embryonic cranial skeleton morphogenesis skeletal system morphogenesis stem cell differentiation positive regulation of multicellular organismal process SMAD protein signal transduction response to sodium phosphate regulation of multicellular organismal development response to mechanical stimulus response to insulin cellular response to zinc ion starvation cellular response to fibroblast growth factor stimulus positive regulation of developmental process cellular response to calcium ion cellular response to parathyroid hormone stimulus cellular response to hyperoxia response to dexamethasone dentinogenesis bone regeneration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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