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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RhoA Antibodies

          AbboMax

          Phospho-Rho (Ser188) Polyclonal Antibody

          View all (29) RhoA antibodies

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          Cite Phospho-Rho (Ser188) Polyclonal Antibody

          • Antibody Testing Data (1)
          Phospho-Rho (Ser188) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-Rho (Ser188) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-Rho (Ser188) Antibody (602-580) in WB

          Western Blot: The cell lysate derived from conditioned MCF-7 was immunoprobed by Rabbit anti-Rho (pS188) (Cat#602-580) at 1:500. An immunoreactive band is observed around ~24 kDa (1). This band is abolished by pre-incubation with immunizing peptide (2). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-Rho (Ser188) Antibody in Western Blot (WB)
          Phospho-Rho (Ser188) Polyclonal Antibody

          Product Details

          602-580

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Dog, Chicken, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of human Rho protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          25mM tris with proprietary stabilizer

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Positive control: Human stomach

          Cellular location: Cell membrane.

          Target Information

          RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho cDNA clone 12; Rho family GTPase; rho1; rho1 GTP-binding protein; RhoA GTPase; small GTP binding protein RhoA; Transforming protein RhoA

          View more View less

          Gene Aliases: ARH12; ARHA; Arha1; Arha2; RHO1; RHO12; RHOA; RHOH12

          View more View less

          UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

          View more View less

          Entrez Gene ID: (Chicken) 395442, (Dog) 403954, (Human) 387, (Rat) 117273, (Mouse) 11848

          View more View less

          Function(s)
          GTPase activity GTP binding protein binding myosin binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding nucleotide binding small GTPase
          Process(es)
          Rho protein signal transduction cell migration regulation of cell migration cytoplasmic microtubule organization actin cytoskeleton reorganization positive regulation of cytokinesis cell junction assembly substrate adhesion-dependent cell spreading Roundabout signaling pathway cleavage furrow formation positive regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly apical junction assembly wound healing, spreading of cells establishment of epithelial cell apical/basal polarity positive regulation of protein serine/threonine kinase activity skeletal muscle satellite cell migration mitotic cleavage furrow formation cellular response to chemokine positive regulation of T cell migration transforming growth factor beta receptor signaling pathway viral process substantia nigra development actin cytoskeleton organization platelet activation regulation of actin cytoskeleton organization regulation of osteoblast proliferation apolipoprotein A-I-mediated signaling pathway positive regulation of NF-kappaB import into nucleus endothelial cell migration ossification involved in bone maturation positive regulation of neuron differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling negative regulation of axonogenesis positive regulation of axonogenesis negative chemotaxis regulation of small GTPase mediated signal transduction positive regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway positive regulation of lipase activity trabecula morphogenesis negative regulation of cell migration involved in sprouting angiogenesis mitotic spindle assembly endothelial tube lumen extension regulation of cell motility cell morphogenesis response to hypoxia regulation of transcription from RNA polymerase II promoter cytoskeleton organization cell adhesion cell-matrix adhesion small GTPase mediated signal transduction skeletal muscle tissue development regulation of actin polymerization or depolymerization metabolic process response to mechanical stimulus response to glucose regulation of neuron projection development cerebral cortex cell migration forebrain radial glial cell differentiation cell differentiation positive regulation of cell growth positive regulation of cell migration androgen receptor signaling pathway positive regulation of actin filament polymerization stress-activated protein kinase signaling cascade negative regulation of intracellular steroid hormone receptor signaling pathway response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to ethanol negative regulation of neuron differentiation positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction positive regulation of smooth muscle contraction neuron projection morphogenesis regulation of dendrite development response to glucocorticoid regulation of calcium ion transport negative regulation of cell death cellular response to progesterone stimulus positive regulation of podosome assembly regulation of neural precursor cell proliferation cell cycle integrin-mediated signaling pathway GTP metabolic process cell division
          It has to be done as per old AB suggested Products section.
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