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          • Primary Antibodies ›
          • c-Kit (CD117) Antibodies

          Invitrogen

          Phospho-c-Kit (Tyr730) Polyclonal Antibody

          Advanced Verification
          2 Published Figures
          4 References
          View all (208) c-Kit (CD117) antibodies

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          Cite Phospho-c-Kit (Tyr730) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (2)
          • Advanced Verification (1)
          Phospho-c-Kit (Tyr730) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-c-Kit (Tyr730) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Phospho-c-Kit (Tyr730) Antibody (44-496G) in IHC (P)

          Immunohistochemistry analysis of Phospho c-kit (Tyr 730), showing staining in the cytoplasm and membrane of paraffin-embedded human gastric carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-c-Kit (Tyr730) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-c-Kit (Tyr730) Antibody in Western Blot (WB)
          Phospho-c-Kit (Tyr730) Antibody in Western Blot (WB)
          Phospho-c-Kit (Tyr730) Antibody in Western Blot (WB)
          Phospho-c-Kit (Tyr730) Antibody in Western Blot (WB)
          Phospho-c-Kit (Tyr730) Antibody
          Phospho-c-Kit (Tyr730) Polyclonal Antibody

          Product Details

          44-496G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:50
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Chicken, Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533666

          Target Information

          KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: belly-spot; c-Kit; c-KIT gene1; c-kit proto-oncogene protein; c-Kit protooncogene; c-kit receptor; c-kit receptor tyrosine kinase; CD117; ckit; dominant spotting; Dominant white spotting; mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; protein kinase; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

          View more View less

          Gene Aliases: Bs; C-Kit; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; MASTC; PBT; SCFR; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

          View more View less

          UniProt ID: (Dog) O97799, (Human) P10721, (Chicken) Q08156, (Mouse) P05532

          View more View less

          Entrez Gene ID: (Bovine) 280832, (Dog) 403811, (Human) 3815, (Chicken) 378783, (Rat) 64030, (Mouse) 16590

          View more View less

          Function(s)
          transmembrane receptor protein tyrosine kinase activity ATP binding cytokine binding metal ion binding nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity stem cell factor receptor activity protein binding kinase activity transferase activity growth factor binding SH2 domain binding protein homodimerization activity receptor signaling protein tyrosine kinase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
          Process(es)
          activation of MAPK activity ovarian follicle development immature B cell differentiation dendritic cell cytokine production inflammatory response spermatogenesis regulation of cell shape peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway lamellipodium assembly T cell differentiation erythrocyte differentiation melanocyte differentiation actin cytoskeleton reorganization mast cell cytokine production embryonic hemopoiesis megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway mast cell degranulation pigmentation digestive tract development stem cell differentiation detection of mechanical stimulus involved in sensory perception of sound cell chemotaxis mast cell differentiation melanocyte migration melanocyte adhesion hematopoietic progenitor cell differentiation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation mast cell chemotaxis positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production glycosphingolipid metabolic process chemotaxis signal transduction transmembrane receptor protein tyrosine kinase signaling pathway spermatid development cell proliferation positive regulation of cell proliferation germ cell migration gonad development visual learning male gonad development response to radiation positive regulation of gene expression programmed cell death cell migration stem cell population maintenance actin cytoskeleton organization hemopoiesis B cell differentiation positive regulation of cell migration positive regulation of pseudopodium assembly positive regulation of mast cell cytokine production somatic stem cell population maintenance ectopic germ cell programmed cell death intracellular signal transduction hematopoietic stem cell migration regulation of cell proliferation positive regulation of tyrosine phosphorylation of STAT protein negative regulation of programmed cell death positive regulation of MAPK cascade tongue development leukocyte activation positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade response to cadmium ion protein autophosphorylation developmental pigmentation positive regulation of long-term neuronal synaptic plasticity epithelial cell proliferation regulation of developmental process positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of developmental process positive regulation of protein kinase B signaling mast cell proliferation positive regulation of mast cell proliferation positive regulation of pyloric antrum smooth muscle contraction regulation of bile acid metabolic process positive regulation of colon smooth muscle contraction positive regulation of small intestine smooth muscle contraction positive regulation of vascular smooth muscle cell differentiation negative regulation of reproductive process multicellular organism development positive regulation of kinase activity positive regulation of MAP kinase activity cellular response to thyroid hormone stimulus myeloid leukocyte differentiation protein phosphorylation signal transduction by protein phosphorylation positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein regulation of developmental pigmentation somatic stem cell division germ cell development phosphorylation cell differentiation
          It has to be done as per old AB suggested Products section.

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