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Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:10-1:50 | - |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Dog, Chicken, Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow. |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Antigen affinity chromatography |
Storage buffer |
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2533666 |
c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: belly-spot; c-Kit; c-KIT gene1; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; CD117; ckit; dominant spotting; Dominant white spotting; mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; protein kinase; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; soluble KIT variant 1; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: Bs; C-Kit; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; PBT; SCFR; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W
UniProt ID: (Dog) O97799, (Human) P10721, (Chicken) Q08156, (Mouse) P05532
Entrez Gene ID: (Bovine) 280832, (Dog) 403811, (Human) 3815, (Chicken) 378783, (Rat) 64030, (Mouse) 16590
Molecular Function:
transmembrane signal receptor
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