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Invitrogen
Phospho-c-Raf (Ser259) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-c-Raf (Ser259) Antibody (44-502) in ICC/IF
Immunofluorescence analysis of Phospho-c-Raf (Ser259) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-c-Raf (Ser259) Polyclonal Antibody (Product # 4... View More
Product Details
44-502
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains serine 259.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; EC 2.7.11.1; kinase Raf1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le
Gene Aliases: c-Raf; CMD1NN; CRAF; NS5; RAF; Raf-1; RAF1
UniProt ID: (Human) P04049
Entrez Gene ID: (Human) 5894
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
kinase activity
small GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
metal ion binding
protein heterodimerization activity
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
response to hypoxia
stimulatory C-type lectin receptor signaling pathway
protein phosphorylation
apoptotic process
signal transduction
activation of adenylate cyclase activity
heart development
cell proliferation
negative regulation of cell proliferation
cell differentiation
platelet activation
thyroid gland development
negative regulation of protein complex assembly
positive regulation of peptidyl-serine phosphorylation
ion transmembrane transport
somatic stem cell population maintenance
regulation of Rho protein signal transduction
insulin secretion involved in cellular response to glucose stimulus
response to muscle stretch
wound healing
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
intermediate filament cytoskeleton organization
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
neurotrophin TRK receptor signaling pathway
thymus development
face development
death-inducing signaling complex assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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