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Invitrogen

Phospho-eNOS (Ser114) Polyclonal Antibody

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Phospho-eNOS (Ser114) Polyclonal Antibody

Product Details

PA5-118638

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NOS3(Accession P29474), corresponding to amino acid residues around phosphorylated Ser114.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903138

Product Specific Information

Antibody detects endogenous levels of eNOS only when phosphorylated at Ser114.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; nitric oxide synthase; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183; unnamed protein product

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Gene Aliases: 2310065A03Rik; ECNOS; eNOS; Nos-3; NOS3

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UniProt ID: (Human) P29474, (Mouse) P70313, (Rat) Q62600

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Entrez Gene ID: (Human) 4846, (Mouse) 18127, (Rat) 24600

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Function(s)
actin monomer binding nitric-oxide synthase activity protein binding calmodulin binding FMN binding superoxide-generating NADPH oxidase activity oxidoreductase activity heme binding tetrahydrobiopterin binding arginine binding cadmium ion binding metal ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding actin binding NADPH-hemoprotein reductase activity iron ion binding beta-catenin binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase protein modifying enzyme
Process(es)
angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis arginine catabolic process nitric oxide biosynthetic process potassium ion transport calcium ion transport mitochondrion organization nitric oxide mediated signal transduction blood circulation regulation of blood pressure negative regulation of cell proliferation response to heat response to hormone negative regulation of platelet activation positive regulation of gene expression negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development regulation of neurological system process lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to fluid shear stress vasodilation negative regulation of potassium ion transport positive regulation of blood vessel endothelial cell migration endothelial cell migration cell redox homeostasis positive regulation of Notch signaling pathway positive regulation of angiogenesis negative regulation of blood pressure tetrahydrobiopterin metabolic process nitric oxide metabolic process negative regulation of smooth muscle cell proliferation homeostasis of number of cells within a tissue establishment of localization in cell negative regulation of calcium ion transport ventricular septum morphogenesis negative regulation of biomineral tissue development regulation of blood vessel diameter negative regulation of extrinsic apoptotic signaling pathway via death domain receptors signal transduction positive regulation of guanylate cyclase activity response to estradiol positive regulation of apoptotic process positive regulation of vasodilation regulation of blood vessel size negative regulation of hydrolase activity oxidation-reduction process cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to cytokine cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
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