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Invitrogen
Phospho-eNOS (Ser1177) Polyclonal Antibody
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Phospho-eNOS (Ser1177) Antibody (PA5-35879) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Phospho-eNOS (Ser1177) was performed in paraffin-embedded mouse duodenum tissue using Phospho-eNOS (Ser1177) Polyclonal Antibody (Product # PA5-35879) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-35879
Applications
Tested Dilution
Publications
Product Specifications
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser1177 of eNOS
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient
RRID
Product Specific Information
Recommended positive controls: Raji, NCI-H929, THP-1, THP-1 (100 nM PMA treatment for 6, 24, 48 hr).
Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Pig (100%), Rabbit (100%), Sheep (100%), Bovine (100%), Guinea pig (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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