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Invitrogen

Phospho-eNOS (Thr495) Polyclonal Antibody

Greener Choice
3 Published Figures
4 References
View all (47) eNOS antibodies

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Phospho-eNOS (Thr495) Polyclonal Antibody

Product Details

PA5-17706

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 4 publications 4 publications

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr495 of human eNOS
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

44 µg/mL

Amount

4.4 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982870

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; Endothelial NOS; eNOS; nitric oxide synthase; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183; unnamed protein product

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Gene Aliases: cNOS; EC-NOS; ECNOS; eNOS; NOS; NOS3; NOSIII

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UniProt ID: (Pig) Q28969, (Human) P29474

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Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846

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Function(s)
nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding actin monomer binding protein binding superoxide-generating NADPH oxidase activity oxidoreductase activity tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding oxidoreductase
Process(es)
arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity oxidation-reduction process cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion positive regulation of blood vessel diameter angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis potassium ion transport calcium ion transport nitric oxide mediated signal transduction blood circulation regulation of blood pressure negative regulation of cell proliferation response to heat response to hormone negative regulation of platelet activation positive regulation of gene expression negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development regulation of neurological system process lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to fluid shear stress vasodilation negative regulation of potassium ion transport positive regulation of blood vessel endothelial cell migration endothelial cell migration cell redox homeostasis positive regulation of Notch signaling pathway positive regulation of angiogenesis negative regulation of blood pressure tetrahydrobiopterin metabolic process nitric oxide metabolic process negative regulation of smooth muscle cell proliferation homeostasis of number of cells within a tissue establishment of localization in cell negative regulation of calcium ion transport ventricular septum morphogenesis negative regulation of biomineral tissue development regulation of blood vessel diameter
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