Immunoprecipitation and Western blot analysis of HEK293 cell lysates treated with UV irradiation using p38 MAPK (phospho Thr180/Tyr182) antibody (Product # PA1-26679) pre-incubated with (1) the peptide immunogen, (2) a generic phosphothreonine-containing peptide, (3) a generic phosphotyrosine-containing peptide, (4) the non-phosphorylated peptide corresponding to the phosphopeptide, (5) no peptide, (6) the phosphopeptide derived from the corresponding region of JNK1+2 and (7) the phosphopeptide derived from the corresponding region of ERK1+2.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human p38 containing threonine 180 and tyrosine 182. This portion is conserved among many speci|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26679 detects p38 (phospho T180 + Y182) from human and rat samples. PA1-26679 is expected to cross react with mouse, canine, monkey, and carp (100% conserved).
PA1-26679 has been successfully used in Western blot procedures.
The PA1-26679 immunogen is a synthetic phosphopeptide corresponding to a portion of human p38 containing threonine 180 and tyrosine 182. This portion is conserved among many species including human, rat, mouse, dog, and carp.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.