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Invitrogen
Pleckstrin Polyclonal Antibody
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FIGURE: 1 / 5
Pleckstrin Antibody (PA5-18225) in ICC/IF
Immunocytochemistry analysis of Pleckstrin using Pleckstrin Polyclonal Antibody (Product # PA5-18225) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed b... View More
Product Details
PA5-18225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (EWIKAIQMASRTGK) corresponding to the C-terminus amino acids of PLEK (aa 341-350).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
Major protein kinase C substrate of platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin; PLEK; unnamed protein product
Gene Aliases: P47; PLEK; PLEK1
UniProt ID: (Human) P08567
Entrez Gene ID: (Human) 5341
hematopoietic progenitor cell differentiation
platelet degranulation
vesicle docking involved in exocytosis
phospholipase C-activating G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
positive regulation of platelet activation
negative regulation of inositol phosphate biosynthetic process
cell projection organization
actin cytoskeleton organization
positive regulation of actin filament depolymerization
phospholipase C-inhibiting G-protein coupled receptor signaling pathway
cortical actin cytoskeleton organization
ruffle organization
positive regulation of actin filament bundle assembly
positive regulation of integrin activation
intracellular signal transduction
inositol phosphate metabolic process
positive regulation of cellular component biogenesis
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of calcium-mediated signaling
regulation of cell diameter
thrombin receptor signaling pathway
negative regulation of thrombin receptor signaling pathway
platelet aggregation
protein secretion by platelet
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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