Invitrogen

Pleckstrin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (29) Pleckstrin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

Pleckstrin Antibody in Immunohistochemistry (IHC)

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Pleckstrin Antibody (PA5-83211) in IHC

Immunohistochemical analysis of Pleckstrin in human bone marrow and pancreas tissues using a Pleckstrin polyclonal antibody (Product # PA5-83211). Corresponding RNA-seq data are presented for the same tissues.

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Pleckstrin Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Pleckstrin demonstrates antibody specificity. Immunohistochemical analysis of Pleckstrin using anti-Pleckstrin Polyclonal Antibody (Product #PA5-83211), shows significant staining of Pleckstrin in bone marrow and shows minimal or weak staining in pancreas tissues. The relative expression levels of Pleckstrin within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-83211

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PLEK

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2790367

Product Specific Information

Immunogen sequence: VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC LLKQGH

Target Information

Major protein kinase C substrate of platelets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin

Gene Aliases: P47; PLEK

UniProt ID: (Human) P08567

Entrez Gene ID: (Human) 5341

Molecular Function: cytoskeletal protein

Function(s)

hematopoietic progenitor cell differentiation platelet degranulation vesicle docking involved in exocytosis integrin-mediated signaling pathway positive regulation of platelet activation negative regulation of inositol phosphate biosynthetic process positive regulation of inositol-polyphosphate 5-phosphatase activity cell projection organization positive regulation of actin filament depolymerization phospholipase C-inhibiting G-protein coupled receptor signaling pathway cortical actin cytoskeleton organization ruffle organization actin cytoskeleton reorganization positive regulation of actin filament bundle assembly positive regulation of integrin activation negative regulation of G-protein coupled receptor protein signaling pathway phosphatidylinositol metabolic process negative regulation of calcium-mediated signaling regulation of cell diameter thrombin receptor signaling pathway platelet aggregation protein kinase C signaling protein secretion by platelet

Process(es)

protein kinase C binding protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding