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Invitrogen
Pleckstrin Polyclonal Antibody
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FIGURE: 1 / 1
Pleckstrin Antibody (PA5-92885) in WB
Western blot analysis of Pleckstrin in extracts of various cell lines. Samples were incubated with Pleckstrin Polyclonal antibody (Product # PA5-92885) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-92885
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human PLEK (NP_0026552)
Conjugate
Form
Liquid
Concentration
2.23 mg/mL
Amount
223 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDTEKGI KELNLEKDKK IFNHCFTGNC VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGAEDPLGAI HLRGCVVTSV ESNSNGRKSE EENLFEIITA DEVHYFLQAA TPKERTEWIR AIQMASRTGK; Positive Samples: THP-1, U-937, Mouse lung, Mouse spleen, Rat spleen
Target Information
Major protein kinase C substrate of platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin; PLEK; unnamed protein product
Gene Aliases: 2010300B13Rik; P47; PLEK; PLEK1
UniProt ID: (Human) P08567, (Mouse) Q9JHK5, (Rat) Q4KM33
Entrez Gene ID: (Human) 5341, (Mouse) 56193, (Rat) 364206
hematopoietic progenitor cell differentiation
platelet degranulation
vesicle docking involved in exocytosis
phospholipase C-activating G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
positive regulation of platelet activation
negative regulation of inositol phosphate biosynthetic process
cell projection organization
actin cytoskeleton organization
positive regulation of actin filament depolymerization
phospholipase C-inhibiting G-protein coupled receptor signaling pathway
cortical actin cytoskeleton organization
ruffle organization
positive regulation of actin filament bundle assembly
positive regulation of integrin activation
intracellular signal transduction
inositol phosphate metabolic process
positive regulation of cellular component biogenesis
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of calcium-mediated signaling
regulation of cell diameter
thrombin receptor signaling pathway
negative regulation of thrombin receptor signaling pathway
platelet aggregation
protein secretion by platelet
positive regulation of inositol-polyphosphate 5-phosphatase activity
actin cytoskeleton reorganization
phosphatidylinositol metabolic process
protein kinase C signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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