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PolH Antibody (A301-231A) in WB
Detection of human PolH by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody (Product # A301-231A) (lot A301-231A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A301-231A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 690 and 713 of Human polymerase (DNA directed), eta.
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
The XPA gene encodes a zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (POL H) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase eta; FLJ16395; FLJ21978; polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein; XP V
Gene Aliases: POLH; RAD30; RAD30A; XP-V; XPV
UniProt ID: (Human) Q9Y253
Entrez Gene ID: (Human) 5429
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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