Bioss

Preproendothelin 1 Polyclonal Antibody

Create Citation

Questions & Answers

Create Citation

Questions & Answers

Product Image Gallery

Preproendothelin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded rat heart muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4°C, DAB staining.

Preproendothelin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded rat stomach labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Preproendothelin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded rat lung labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Preproendothelin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ET-1 Polyclonal Antibody (bs-0188R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Preproendothelin 1 Antibody in Western Blot (WB) — Lane 1: mouse liver lysates; Lane 2: rat liver lysates probed with Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Western Blot (WB)

1:300

-

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Preproendothelin 1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Target Information

Endothelins are endothelium-derived vasoconstrictor peptides.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: Big endothelin-1; Endothelin-1; ET-1; HAST-I; N-hydroxyarylamine sulfotransferase; N-hydroxylarylamine sulfotransferase; Phenol sulfotransferase 1c1; PPET1; preproendothelin; Preproendothelin-1; ST1C1; ST1C3; Sulfotransferase 1C1; Sulfotransferase 1C3; sulfotransferase family 1A phenol-preferring member 2; sulfotransferase family 1A, member 2; sulfotransferase family 1A, phenol-preferring, member 2; sulfotransferase family, cytosolic, 1C, member 1; sulfotransferase family, cytosolic, 1C, member 3; sulfotransferase, phenol preferring 2; sulphotransferase family cytosolic 1A phenol-preferring member 2

Gene Aliases: ARCND3; EDN1; ET-1; ET1; HAST-I; HDLCQ7; PPET1; preproET; QME; St1c1; ST1C3; Stp2; Sult1a2; Sult1c1; SULT1C3

UniProt ID: (Human) P05305, (Rat) P22388, (Mouse) P22387, (Human) Q6IMI6, (Rat) P50237

Entrez Gene ID: (Human) 1906, (Rat) 24323, (Mouse) 13614, (Human) 442038, (Rat) 65185

Molecular Function: transferase

negative regulation of transcription from RNA polymerase II promoter prostaglandin biosynthetic process patterning of blood vessels in utero embryonic development histamine secretion regulation of systemic arterial blood pressure by endothelin regulation of pH cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration protein kinase C-activating G-protein coupled receptor signaling pathway cell-cell signaling heart development respiratory gaseous exchange body fluid secretion positive regulation of cell proliferation dorsal/ventral pattern formation response to ozone multicellular organism aging positive regulation of heart rate positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of receptor biosynthetic process neural crest cell development phosphatidylinositol 3-kinase signaling response to activity artery smooth muscle contraction vein smooth muscle contraction glucose transport cell growth regulation of vasoconstriction sensory perception of pain calcium-mediated signaling peptide hormone secretion nitric oxide transport negative regulation of blood coagulation positive regulation of cell migration neutrophil chemotaxis negative regulation of cAMP biosynthetic process phospholipase D-activating G-protein coupled receptor signaling pathway negative regulation of cellular protein metabolic process positive regulation of prostaglandin secretion response to lipopolysaccharide response to testosterone negative regulation of smooth muscle cell apoptotic process response to prostaglandin F response to nicotine cellular response to drug positive regulation of urine volume positive regulation of renal sodium excretion response to muscle stretch epithelial fluid transport vasoconstriction protein kinase C deactivation middle ear morphogenesis positive regulation of odontogenesis superoxide anion generation rhythmic excitation response to amino acid positive regulation of MAP kinase activity positive regulation of JUN kinase activity response to leptin leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of cell size positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of hormone secretion negative regulation of hormone secretion inositol phosphate-mediated signaling positive regulation of smooth muscle cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity cartilage development positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of nitric-oxide synthase biosynthetic process membrane depolarization regulation of sensory perception of pain maternal process involved in parturition positive regulation of sarcomere organization positive regulation of prostaglandin-endoperoxide synthase activity positive regulation of cell growth involved in cardiac muscle cell development positive regulation of chemokine-mediated signaling pathway cellular response to calcium ion cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus cellular response to glucocorticoid stimulus cellular response to mineralocorticoid stimulus cellular response to fatty acid cellular response to hypoxia response to dexamethasone cellular response to transforming growth factor beta stimulus response to salt skeletal system development response to hypoxia regulation of blood pressure intracellular signal transduction response to drug blood vessel morphogenesis response to transforming growth factor beta positive regulation of neutrophil chemotaxis regulation of blood vessel size sulfur compound metabolic process metabolic process

Process(es)

cytokine activity hormone activity protein binding endothelin A receptor binding endothelin B receptor binding receptor binding alcohol sulfotransferase activity aryl sulfotransferase activity