Immunofluorescent analysis of Prodynorphin using a Prodynorphin polyclonal antibody (Product # PA1-30935). Systemic artemin normalized immunohistochemical markers in the ipsilateral L5 DRG, dorsal spinal cords and sciatic nerves of SNL rats. Immunofluorescent labeling was done 14 d after sham or SNL surgery and treatment with vehicle or artemin starting on day 3 after surgery. Scale bar = 100 um for DRG (first two rows), 50 m for sciatic nerve (third row) and 200 m for spinal cord (bottom row).
|Tested species reactivity||Rat|
|Host / Isotype||Guinea pig / IgG|
|Immunogen||SQENPNTYSEDLCVCorresponding to residues 245-248 of rat proDynorphin|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30935 detects proDynorphin from rat samples.
PA1-30935 has been successfully used in immunohistochemistry applications.
The PA1-30935 immunogen is: SQENPNTYSEDLCVCorresponding to residues 245-248 of rat proDynorphin.
expression in hippocampus increases in response to traumatic brain injury; may play a role in regulatio of neurotransmitter release.
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