Proteintech

Profilin 1 Polyclonal Antibody

View all (17) Profilin 1 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 11680-1-AP (Profilin 1 antibody) at dilution of 1:50 (under 10x lens).

Profilin 1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Profilin 1 Fusion Protein Ag2286

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actin binding protein; Epididymis tissue protein Li 184a; Profilin I; Profilin-1

Gene Aliases: ALS18; Pfn; PFN1

UniProt ID: (Human) P07737, (Rat) P62963, (Mouse) P62962

Entrez Gene ID: (Human) 5216, (Rat) 64303, (Mouse) 18643

Molecular Function: actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

neural tube closure positive regulation of epithelial cell migration actin cytoskeleton organization negative regulation of actin filament polymerization positive regulation of actin filament polymerization negative regulation of actin filament bundle assembly positive regulation of actin filament bundle assembly positive regulation of ATPase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription protein stabilization positive regulation of DNA metabolic process positive regulation of stress fiber assembly negative regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway cellular response to growth factor stimulus cell-cell adhesion positive regulation of ruffle assembly regulation of transcription from RNA polymerase II promoter actin polymerization or depolymerization response to organic substance regulation of actin polymerization or depolymerization sequestering of actin monomers

Process(es)

adenyl-nucleotide exchange factor activity actin binding actin monomer binding receptor binding protein binding phosphatidylinositol-4,5-bisphosphate binding Rho GTPase binding poly(A) RNA binding proline-rich region binding cadherin binding involved in cell-cell adhesion