Proteintech

Profilin 1 Polyclonal Antibody

View all (18) Profilin 1 antibodies

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Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 11680-1-AP (Profilin 1 antibody) at dilution of 1:50 (under 10x lens).

Profilin 1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Profilin 1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11680-1-AP (Profilin 1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Product Details

11680-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Profilin 1 Fusion Protein Ag2286 (1-140 aa encoded by BC006768) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.37 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAGWNAYID NLMADGTCQD AAIVGYKDSP SVWAAVPGKT FVNITPAEVG VLVGKDRSSF YVNGLTLGGQ KCSVIRDSLL QDGEFSMDLR TKSTGGAPTF NVTVTKTDKT LVLLMGKEGV HGGLINKKCY EMASHLRRSQ Y (1-140 aa encoded by BC006768)

Target Information

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

PloS one

Ma B,Chen J,Mu Y,Xue B,Zhao A,Wang D,Chang D,Pan Y,Liu J

Mon Feb 11 00:00:00 EST 2019

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Journal of experimental & clinical cancer research : CR

Lian Y,Xiong F,Yang L,Bo H,Gong Z,Wang Y,Wei F,Tang Y,Li X,Liao Q,Wang H,Zhou M,Xiang B,Wu X,Li Y,Li X,Chen X,Li G,Guo C,Zeng Z,Xiong W

Tue Oct 16 00:00:00 EDT 2018

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zeng Z,Bo H,Gong Z,Lian Y,Li X,Li X,Zhang W,Deng H,Zhou M,Peng S,Li G,Xiong W

Fri Jan 01 00:00:00 EST 2016

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Oncotarget

Bo H,Gong Z,Zhang W,Li X,Zeng Y,Liao Q,Chen P,Shi L,Lian Y,Jing Y,Tang K,Li Z,Zhou Y,Zhou M,Xiang B,Li X,Yang J,Xiong W,Li G,Zeng Z

Fri Aug 21 00:00:00 EDT 2015

The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function.

Biomaterials

Yang D,Lü X,Hong Y,Xi T,Zhang D

Fri Aug 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: actin binding protein; Epididymis tissue protein Li 184a; Profilin I; Profilin-1

Gene Aliases: ALS18; Pfn; PFN1

UniProt ID: (Human) P07737, (Rat) P62963, (Mouse) P62962

Entrez Gene ID: (Human) 5216, (Rat) 64303, (Mouse) 18643

Molecular Function: actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

neural tube closure positive regulation of epithelial cell migration actin cytoskeleton organization negative regulation of actin filament polymerization positive regulation of actin filament polymerization negative regulation of actin filament bundle assembly positive regulation of actin filament bundle assembly positive regulation of ATPase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription protein stabilization positive regulation of DNA metabolic process positive regulation of stress fiber assembly negative regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway cellular response to growth factor stimulus cell-cell adhesion positive regulation of ruffle assembly regulation of transcription from RNA polymerase II promoter actin polymerization or depolymerization response to organic substance regulation of actin polymerization or depolymerization sequestering of actin monomers

Process(es)

adenyl-nucleotide exchange factor activity actin binding actin monomer binding receptor binding protein binding phosphatidylinositol-4,5-bisphosphate binding Rho GTPase binding poly(A) RNA binding proline-rich region binding cadherin binding involved in cell-cell adhesion