Invitrogen

Progesterone Receptor Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (134) Progesterone Receptor antibodies

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Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Progesterone Receptor was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor Polyclonal Antibody (Product # PA5-68778) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents SK-BR-3 cells having no expression of Progesterone Receptor. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Progesterone Receptor Antibody in Western Blot (WB) — Western blot analysis of Progesterone-Receptor on mouse thymus tissue. The sample was probed with a Progesterone-Receptor polyclonal antibody (Product # PA5-68778) using a primary antibody dilution of 0.2-1.0 µg/mL.

Progesterone Receptor Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Progesterone Receptor Polyclonal Antibody (Product # PA5-68778), shows positive Nuclear localization in T-47D when compared to SK-BR-3 cells. {RE}

Product Details

PA5-68778

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1.0 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	synthetic peptide directed towards the middle region of mouse Progesterone Receptor
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2689394

Product Specific Information

This target displays homology in the following species: Dog: 91%; Guinea Pig: 91%; Mouse: 100%; Rat: 91%

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.