AbboMax

Progesterone Receptor (PR) Polyclonal Antibody

View all (134) Progesterone Receptor antibodies

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Progesterone Receptor (PR) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunohistochemistry: Human breast cancer tissue stained with Rabbit Anti-PR antibody, (Cat# 500-2074) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. .

Progesterone Receptor (PR) Antibody in Western Blot (WB) — Western Blot: The whole lysate derived from PC3 (20 µg/lane) was immunoprobed by Rabbit anti-PR (Cat#500-2074) at 1:500. Observed a major immunoreactive band at molecular weight ~100 kDa.

Product Details

500-2074

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	2-10 µg/mL	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	2-10 µg/mL	-

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to C-terminus of human Progesterone receptor View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with proprietary stabilizer
Contains	0.01% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Positive control: Breast Cancer Tissue

Cellular location: Nucleus, Cytoplasm.

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor; steroid hormone receptor

Gene Aliases: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B

UniProt ID: (Human) P06401, (Rat) Q63449, (Mouse) Q00175

Entrez Gene ID: (Bovine) 100848466, (Human) 5241, (Rat) 25154, (Mouse) 18667

ovulation from ovarian follicle luteinization epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of gene expression positive regulation of smooth muscle cell migration diterpenoid metabolic process intracellular receptor signaling pathway mammary gland development response to progesterone response to cocaine negative regulation of apoptotic process steroid hormone mediated signaling pathway response to estrogen estrous cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of epithelial cell proliferation progesterone receptor signaling pathway female mating behavior tertiary branching involved in mammary gland duct morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus cellular response to luteinizing hormone stimulus transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling paracrine signaling lung alveolus development

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding hormone binding sequence-specific DNA binding lipid binding metal ion binding