Recommended positive controls: 293T, Daudi, human B lymphoblast, Jurkat.
Predicted reactivity: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%.
PA1-24426 has been successfully used in Western Blot procedures dilute this antibody with 5% skim milk/PBS buffer.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Protein Aliases: epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; Prohibitin
Gene Aliases: HEL-215; HEL-S-54e; PHB; PHB1
UniProt ID: (Human) P35232
Entrez Gene ID: (Human) 5245