|Immunohistochemistry (IHC)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide region of of human PHB.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 2% sucrose|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-24426 detects Prohibitin (PHB) in Human samples.
PA1-24426 has been successfully used in Western Blot procedures dilute this antibody with 5% skim milk/PBS buffer.
PA1-24426 immunogen corresponds to Polyclonal antibody from rabbits immunized with a synthetic peptide region of of human PHB.
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; PHB; Prohibitin
Gene Aliases: HEL-215; HEL-S-54e; PHB; PHB1
UniProt ID: (Human) P35232
Entrez Gene ID: (Human) 5245
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