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Invitrogen
Prom1 Monoclonal Antibody (2F8C5)
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FIGURE: 1 / 9
Prom1 Antibody (MA1-219) in ICC/IF
Immunofluorescent analysis of Prom1 (green) in HT-29 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Prom1 mouse monoclonal antibody (Product # MA1-219) at a dilution of 1:100 in blocking buffer overnight at 4°C, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:1000 for at le... View More
Product Details
MA1-219
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cynomolgus monkey,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F8C5
Immunogen
Purified recombinant fragment of human Prom1 (AA:20-108) expressed in E.Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-219 Prom1 antibody detects Prom1 (CD133) by western blot and immunofluorescence.
MA1-219 Prom1 antibody detects Prom1 by western blot at ~130 kDa.
Target Information
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-transmembrane cell surface receptor; AC133 antigen; Antigen AC133; antigen CD133; CD107b; CD133; hematopoietic stem cell antigen; hematopoitic stem cell surface marker; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; membrane glycoprotein; Prominin-1; Prominin-like protein 1; Prominin1; Retinitis pigmentosa 41, Cone-rod dystrophy 12; unnamed protein product
Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4
UniProt ID: (Human) Q6SV51
Entrez Gene ID: (Human) 8842
retina layer formation
photoreceptor cell maintenance
retina morphogenesis in camera-type eye
camera-type eye photoreceptor cell differentiation
glomerular visceral epithelial cell differentiation
glomerular parietal epithelial cell differentiation
positive regulation of nephron tubule epithelial cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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