Western blot detection of Protein an In (lane 1) Protein A Reduced, (lane 2) Protein A Reduced, (lane 3) Protein A Non-Reduced, (lane 4) Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Samples were probed using a Protein A polyclonal antibody (Product # PA1-26853) at a dilution of 1:5,000 following incubation overnight at 4°C and detected with a DyLight 649 goat secondary antibody at 1:10,000 for 90 min at room temperature. Samples were blocked 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.
|Tested species reactivity||Bacteria|
|Host / Isotype||Goat / IgG|
|Immunogen||Protein A from Staphylococcus aureus.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26853 detects Protein A.
PA1-26853 has been successfully used in immunohistochemistry (frozen), ELISA, and Western blot procedures.
The PA1-26853 immunogen is protein A from Staphylococcus aureus.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
S. aureus is a Gram positive, sperical bacterium (coccus) which on microscopic examination appears in pairs, short chains, or bunched, grape like clusters. The bacterium forms characteristic golden yellow colonies on blood agar cultures. It is part of the normal flora of humans and is found on nasal passages, skin and mucous membranes. It is an opportunistic pathogen of humans, causes a wide range of suppurative infections, as well as food poisoning, septicaemia and toxic shock syndrome. S. aureus has become resistant to many commonly used antibiotics; some strains are now resistant to vancomycin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.