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Product Details
P200P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The purified peptide conjugated to KLH.
Conjugate
Form
Liquid
Concentration
0.5-2 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4 C, not refrozen, and preferably used the same day.
In immunoelectrophoresis use 2 ul serum, plasma or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.
Target Information
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ42306; IA; MGC9201; Mpp alpha; PP2C-alpha; PP2C-beta; Protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase 1B; protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform; protein phosphatase 1B, magnesium dependent, beta isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; Protein phosphatase IA; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform; Protein phosphatase type 1B (formely 2C) Mg-dependent beta isoform; Protein phosphatase type 1B (formely 2C), Mg-dependent, beta isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A; protein phosphatase, Mg2+/Mn2+ dependent, 1B; Ser/Thr protein phosphatase type 2C beta 2 isoform
Gene Aliases: 2310003C21Rik; 2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1; PP2C-ALPHA; PP2C-beta; PP2C-beta-X; Pp2c1; Pp2c2; PP2CA; PP2Calpha; PP2CB; PP2CBETA; PPC2BETAX; PPM1A; PPM1B; PPPM1A; Pppm1b
UniProt ID: (Human) P35813, (Bovine) O62829, (Bovine) O62830, (Human) O75688, (Mouse) P49443, (Rat) P20650, (Mouse) P36993, (Rat) P35815
Entrez Gene ID: (Human) 5494, (Bovine) 281994, (Bovine) 281995, (Human) 5495, (Mouse) 19042, (Rat) 24666, (Mouse) 19043, (Rat) 24667
magnesium ion binding
protein serine/threonine phosphatase activity
signal transducer activity
protein binding
manganese ion binding
calmodulin-dependent protein phosphatase activity
R-SMAD binding
phosphoprotein phosphatase activity
catalytic activity
protein C-terminus binding
hydrolase activity
metal ion binding
protein dephosphorylation
N-terminal protein myristoylation
negative regulation of SMAD protein complex assembly
Wnt signaling pathway
dephosphorylation
positive regulation of Wnt signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
peptidyl-threonine dephosphorylation
negative regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
positive regulation of protein export from nucleus
cellular response to transforming growth factor beta stimulus
negative regulation of interferon-beta production
negative regulation of defense response to virus
negative regulation of transcription from RNA polymerase II promoter
cell cycle arrest
negative regulation of NIK/NF-kappaB signaling
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