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Invitrogen
Prothrombin Polyclonal Antibody
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FIGURE: 1 / 1
Prothrombin Antibody (PA5-86933) in WB
Western blot analysis of Prothrombin in Lane 1: K562 whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: L02 whole cell lysate (40 µg), Lane 4: Liver tissue lysate of rat (40 µg), Lane 5: Liver tissue lysate of mouse (40 µg). Sample was incubated with Prothrombin polyclonal antibody (Product # PA5-86933) using a 1:500 dilution.
Product Details
PA5-86933
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 430-480 of Human Prothrombin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
Prothrombin or Coagulation factor II, is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coagulation factor 2; Coagulation factor II; prepro-coagulation factor II; Prothrombin; prothrombin B-chain; thrombin; thrombin factor II; thrombin precursor; unnamed protein product
Gene Aliases: Cf-2; Cf2; F2; FII; PT; RPRGL2; THPH1
UniProt ID: (Mouse) P19221, (Human) P00734
Entrez Gene ID: (Mouse) 14061, (Rat) 29251, (Human) 2147
serine-type endopeptidase activity
receptor binding
calcium ion binding
peptidase activity
thrombospondin receptor activity
lipopolysaccharide binding
endopeptidase activity
protein binding
growth factor activity
heparin binding
serine-type peptidase activity
hydrolase activity
receptor agonist activity
protease
serine protease
protein modifying enzyme
positive regulation of protein phosphorylation
proteolysis
acute-phase response
cell surface receptor signaling pathway
blood coagulation
positive regulation of cell proliferation
regulation of cell shape
response to wounding
regulation of gene expression
positive regulation of phosphatidylinositol 3-kinase signaling
response to inactivity
platelet activation
positive regulation of blood coagulation
positive regulation of cell growth
positive regulation of collagen biosynthetic process
wound healing
fibrinolysis
negative regulation of proteolysis
negative regulation of astrocyte differentiation
positive regulation of release of sequestered calcium ion into cytosol
regulation of cytosolic calcium ion concentration
cellular response to mechanical stimulus
positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of reactive oxygen species metabolic process
G-protein coupled receptor signaling pathway
hemostasis
negative regulation of platelet activation
regulation of blood coagulation
negative regulation of blood coagulation
positive regulation of insulin secretion
positive regulation of JAK-STAT cascade
regulation of body fluid levels
cytolysis by host of symbiont cells
positive regulation of protein kinase B signaling
negative regulation of fibrinolysis
antimicrobial humoral immune response mediated by antimicrobial peptide
thrombin receptor signaling pathway
neutrophil mediated killing of gram-negative bacterium
negative regulation of cytokine production involved in inflammatory response
positive regulation of protein localization to nucleus
ligand-gated ion channel signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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